Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2015/03/05

Project name

Main project page Previous entry      Next entry

03/05/2015 Today I am doing a digestion of the KAH 126 plasmid that was mini-prepped on 1/28/2015 (in CRISPR notebook). Here are the contents used: Reaction 1 Thing Vol 0.5ug Plasmid 1.1 uL EcoRI 0.5 uL PstI 0.5 uL Green digest buffer 1 uL Water 6.9 uL Reaction 2 Thing Vol 0.5ug Plasmid 1.23 uL EcoRI 0.5 uL PstI 0.5 uL Green digest buffer 1 uL Water 6.77 uL After pipetting each thing into a PCR tube, I ran the product on a gel to see if there was any cutting. This will allow us to conclude whether or not the plasmid is what we think it is.