Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2013/10/15

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mm/dd/yyyy

Recut more J06702 for 55 minutes.

Colonies on background plate likely from uncut vector.

Gel purified.
Also ran PCR products from yesterday
Gel results

Lane 1 Lane 2 Lane 3 Lane 4 Lane 5
Ladder LuxI LasI LasR pLas J06702
No band 1 correct band 1 correct band no band 1 correct band


Extracted J06702 from gel. 44ng/μl.