Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2013/10/10

From OpenWetWare
Jump to: navigation, search
Owwnotebook icon.png Project name Report.pngMain project page
Resultset previous.pngPrevious entry      Next entryResultset next.png

mm/dd/yyyy

Spun all cells in 15mL conicals at 2000RCF
Combined triplicates

OD650 GFP
LuxR 1 0.773 0.7963
LuxR 2 0.768 8088
RhlI 0.706 1579
EsaI 0.792 2597


Resuspended 30mL culuter into 3mL total

1.5mLResidual sup+pellet
1.5mL=new LB+amp
Measured by pipetting into a new tube


1mL=100uL, add 300ul into each tube
2:30PM
Started shaking

Took measurements every hour-ish by pulling 100ul from each tube and measuring GFP and OD650
7PM

8PM

9PM

10PM

11:30PM



another one tomorrow at 10AM

Ligation pLac-RBS-into J06702

tons of colonies on all plates, including backbone. Maybe because I didn't gel purify the vector, I could have single cut? Or uncut?