mm/dd/yyyy
Spun all cells in 15mL conicals at 2000RCF
Combined triplicates
|
OD650 |
GFP
|
| LuxR 1 |
0.773 |
0.7963
|
| LuxR 2 |
0.768 |
8088
|
| RhlI |
0.706 |
1579
|
| EsaI |
0.792 |
2597
|
Resuspended 30mL culuter into 3mL total
- 1.5mLResidual sup+pellet
- 1.5mL=new LB+amp
- Measured by pipetting into a new tube
1mL=100uL, add 300ul into each tube
2:30PM
Started shaking
Took measurements every hour-ish by pulling 100ul from each tube and measuring GFP and OD650
7PM
8PM
9PM
10PM
11:30PM
another one tomorrow at 10AM
Ligation pLac-RBS-into J06702
- tons of colonies on all plates, including backbone. Maybe because I didn't gel purify the vector, I could have single cut? Or uncut?
| 
Project name
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