Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2013/09/28

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mm/dd/yyyy

8am

Sender/Receiver OD650 GFP RFP
EsaI 0.679 1500
0.671 1545
0.634 1402
RhlI 0.755 13497
0.792 14323
0.763 13992
Ctrl 0.736 6645
0.712 6485
0.740 6598
LuxR-GFP 0.548 1619
0.563 1858
0.576 1957
LuxR-RFP 0.576 768
0.564 682
0.562 694


Combined 3-5mL cultures of each sender into 15mL conicals. Spun in Wang lab large centrifuge
spun receiver cells in 2mL tubes at 12k g for 5min
I didn't write this down but I'm assuming I split the receiver cells into the sender supernatant in singlet.