07/19/2013
Ligation/transformation plates have colonies but they're all in the middle. Same with the backbone control. Picked colonies just in case.
I03522 or whatever has colonies. Picked two.
Started the oligo annealing over again. I shouldn't have cut or dephosphorylated the oligos. That was dumb.
Miniprepped the colonies I picked last night. Tried splitting the 3ml cultures into 2-1.5ml cultures to mini prep. Still got low yields.
| Part |
Size |
Miniprep yield 1 |
Miniprep yield 2
|
| K092900+cyan |
~2900 |
|
| F2620+GFP |
~2000 |
|
| K082035 |
|
6 |
8
|
| K084007 |
835 |
|
Cut a bunch of QS plasmids from the last few months with X/P to see which ones might be worth transforming and growing up to PCR out parts for the expression vectors. Ran on a gel
| No. |
Name |
Length |
Gel check
|
| 1 |
LuxR-GFP Receiver |
1900 |
+
|
| 2 |
K092900 + E0240 |
2200 |
-
|
| 3 |
C0061 - 1 |
643 |
couldn't tell
|
| 4 |
C0061 - 2 |
643 |
couldn't tell
|
| 5 |
C0062 |
781 |
couldn't tell
|
| 6 |
F2620 (81ng/ul) |
1061 |
+
|
| 7 |
F2620 (52ng/ul) |
1061 |
+
|
| 8 |
K091134 |
2048 |
-
|
| 9 |
K092900 |
2102 |
_
|
| 10 |
K145254 |
862 |
+
|
| 11 |
K082035 |
884 |
+
|
| 12 |
I0466 |
942 |
+
|
| 13 |
K084007 |
835 |
+
|
| 14 |
RBS-LasI |
600 |
+
|
| 15 |
messed up |
? |
-
|
| 16 |
K092900-cyan |
2900 |
-
|
| 17 |
F2620-GFP |
2000 |
-
|
| 18 |
K084007 |
835 |
+
|
| 19 |
RBS-LuxI |
798 |
not sure
|
| 20 |
J06702 56ng/ul |
869 |
+
|
Transformed each of the positive ones and K091134 because I don't have another LasR option.
Transformed the second annealing reaction.
| 
Project name
Main project page
Previous entry Next entry
