Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2013/03/09

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Entry title

miniprepped RBS+LuxI and RBS+LasI
cut (all minipreps, some from before, colony numbers on gel) for 15 minutes at 37deg and ran a gel right away:

[[image: ‎ |400px|digests]]

Lane label Part number Restriction enzymes expected length
1 B0034+C0061 X & P 661 & 2000ish
2 cB0034+C0078 X & P 685 & 2000ish
3 K082035 X&P 884 & 2000ish
4 K084007 X & P 835 & 2000ish
5 K145254 X & P 862 & 2079
6 K092100 S & P 2909ish
7 K091134 X & P 2048 & 2000ish