Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2013/03/07

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Entry title

minipreps

streak

cut and extract and clone

pull out promoters + RBS from registry

gel extract and ligate and transform part 6


miniprep C0061 1 and 2 C0062 K081016 that haynes grew up, streaked on plate (she found by PCR) R0071 that haynes grew up, streaking on plate " "

cut K081016 and R0071 from above, ran on a gel, didn't work

Ernesto ran colony PCR on a bunch of mini preps and colonies

transformed RBS+LuxI and RBS+LasI

Part 260 280 260/280 ng/ul
K079021 0.131 0.07 1.836 130.892
I13522 0.125 0.068 1.836 125.239
K079020 0.086 0.046 1.855 85.913
J45200 0.081 0.045 1.81 80.868
J04450 0.071 0.038 1.859 70.782