05/24/2015
Added RNase to g025 sonicated chromatin, 30min at 37
finally got enough DNA from PCR for g025 3. Means there's probably something wrong with my Phusion.
| Cell type
|
gRNA
|
rep
|
260
|
280
|
260/280
|
ng/µL
|
ul for 400ng
|
ul hyb buff
|
ul H2O
|
| Luc14 |
g025 |
3 |
0.034 |
0.019 |
1.809 |
33.885 |
11.80 |
1.50 |
1.70
|
Can run surveyor on g025 and 31 today. Set up annealing reaction:
| Cell type
|
gRNA
|
rep
|
260
|
280
|
260/280
|
ng/µL
|
ul for 400ng
|
ul hyb buff
|
ul water
|
| Luc14 |
25 |
1 |
0.035 |
0.02 |
1.792 |
35.279 |
11.34 |
1.50 |
2.16
|
| Luc14 |
25 |
2 |
0.046 |
0.025 |
1.876 |
46.254 |
8.65 |
1.50 |
4.85
|
| Luc14 |
25 |
3 |
0.034 |
0.019 |
1.809 |
33.885 |
11.80 |
1.50 |
1.70
|
|
|
|
|
|
|
|
|
|
|
| Luc14 |
31 |
1 |
0.07 |
0.037 |
1.9 |
69.982 |
5.72 |
1.50 |
7.78
|
| Luc14 |
31 |
2 |
0.087 |
0.046 |
1.885 |
86.915 |
4.60 |
1.50 |
8.90
|
| Luc14 |
31 |
3 |
0.035 |
0.019 |
1.868 |
35.484 |
11.27 |
1.50 |
2.23
|
Added proteinase k to 25 sonicated chromatin. 62 for 2 hours
conc of sheared chromatin
| Cells
|
gRNA
|
rep
|
260
|
280
|
260/280
|
ng/µL
|
ul for 50ug
|
ul dil buff
|
| Luc14 |
25 |
1 |
2.272 |
1.32 |
1.721 |
2271.864 |
22.01 |
177.99
|
| Luc14 |
25 |
2 |
2.426 |
1.42 |
1.708 |
2426.165 |
20.61 |
179.39
|
| Luc14 |
25 |
3 |
2.022 |
1.17 |
1.728 |
2021.664 |
24.73 |
175.27
|
| Gal4 |
25 |
1 |
2.128 |
1.267 |
1.68 |
2127.688 |
23.50 |
176.50
|
| Gal4 |
25 |
2 |
1.305 |
0.795 |
1.643 |
1305.148 |
38.31 |
161.69
|
| Gal4 |
25 |
3 |
1.903 |
1.138 |
1.672 |
1902.941 |
26.28 |
173.72
|
| 
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