Haynes Lab:Notebook/CRISPR Editing/2016/03/12
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03/12/2016Colony PCR of colonies on ligation plates from Saturday. Primers P220/221 will give a 1240bp band if GFP is inserted. will give a 439bp for backbone only. Will be slightly longer if the backbone was only cut once. Used 2x taq MM. NEB suggests an annealing T of 51°C with Taq, will do 55°C to try to avoid lots of off target binding to gDNA.
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