Haynes Lab:Notebook/CRISPR Editing/2015/08/17

Project name

Main project page Previous entry      Next entry

08/17/2015 Phusion PCR for singlet full coverage of all gRNAs tested gRNA F primer R primer 29 P149 P174 30 P159 P215 33 P149 P176 36 P149 P174 40 P163 P160 41 P175 P214 42 P213 P214 43 P213 P214 Found some triplicate samples of Luc14 and Gal4-EED treated samples for g023, g044, g045. PCR'd with P197/198. Checked on a gel, negative controls blank. Made 1:1000 dilutions and ran nested PCRd with the following primers: gRNA F primer R primer 23 P149 P215 44 P213 P214 45 P151 P160