Haynes Lab:Notebook/CRISPR Editing/2015/05/17
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Added 1ul enhancer and 1ul surveyor enzyme to the 11 tubes I annealed yesterday. 2ul of water to the surveyor negative tubes. incubated at 42°C for 60 mins, added 2.5ul stop solution, ran on bioanalyzer
Purified the digests with Qiaquick PCR purification kit. Eluted in 30ul. Will retry the ligation tomorrow. Will order more of the zymo competent cell mix
Should recut more backbone, do like 2-3 reactions for a total of 2-3ug, column purify. Then cut the insert and dephos it. Should also make sure the EcoRI works in cutsmart buffer.