Haynes Lab:Notebook/CRISPR Editing/2015/05/06

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05/06/2015

purified nested PCR of off-target gRNAs from Luc14

Sample Read# 260 280 260/280 ng/µL ul for 400ng
g051 0.052 0.027 1.911 51.547 7.76
g052 0.058 0.031 1.85 58.161 6.88
g053 0.071 0.038 1.851 71.088 5.63
g054 0.065 0.035 1.855 65.16 6.14
g055 0.088 0.047 1.874 87.619 4.57
g056 0.061 0.035 1.737 61.227 6.53