Haynes Lab:Notebook/CRISPR Editing/2015/02/04

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02/04/2015

JW: analyzed sequencing results for KAH126. Sequencing failed for both minipreps for both primers.
Possible reasons

Primers don't match the backbone
Primers are not what we think they are
backbone isn't what we think it is
Miniprepped colonies are just genomic DNA
Sequencing run failed