Haynes Lab:Notebook/CRISPR Editing/2014/09/30

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09/30/2014

Annealed 4 reactions each of

' ul for 400ng ul elution solution ul water ul 10x annealing buffer
gRNA 20 Luc 2.86 0.14 6 1
gRNA 20 +dox 1 1.96 1.04 6 1
gRNA 20 +dox 2 2.23 0.77 6 1
gRNA 29 Luc 2.52 0.48 6 1
gRNA 29 +dox 1 2.61 0.39 6 1
gRNA 29 +dox 2 2.04 0.96 6 1
Luc14 3.05 -0.05 6 1


Added two of each with 0.5ul Surveyor and 1ul enhancer, added 1.5ul of water to the other two of each, incubate at 42deg C for 45min.
Ran one of each on a gel