Haynes Lab:Notebook/ASU iGEM/2012/06/26
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6-26-12
- Transformation:
- Transformed DNA:
- double terminator (B0017, 2:6K)
- T7 RNA polymerase (I715038, 2:15C)
- puc19, negative control
- Protocol from: http://tools.invitrogen.com/content/sfs/manuals/subcloningefficiencydh5alpha_man.pdf (30 minute transformation)
- Cells: dh5a