Harvard:Biophysics 101/Notebook:ZS/2007-2-20
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Assignment 3, Due 2/20
#Zachary Sun, Assignment 3 (alpha edition) #2.20.07, final version forthcoming #!/usr/bin/env python import os from Bio import Clustalw from Bio import GenBank, Seq from Bio.Seq import Seq,translate cmdline = Clustalw.MultipleAlignCL(os.path.join(os.curdir, 'apoe.fasta')) cmdline.set_output('test.aln') align = Clustalw.do_alignment(cmdline) #Zach's addition: Looking to see if the mutation is in intron or exon; #sorry it is a poor implementation, but hopefully will expand on this #in upcoming week (could not get implementation working for a while =( ##Determining start and end sites assuming first is reference coding seq refSeqObj = align.get_all_seqs() refSeq = refSeqObj[0].seq.tostring() start_site = refSeq.find('ATG') #position of start codon counter = start_site countcodon = 0; for i in range(len(refSeq)-4-start_site): #to determine stop codon point totrans = totrans + refSeq[counter] counter = counter + 1 stoptest = refSeq[counter]+refSeq[counter+1]+refSeq[counter+2] if countcodon == 2: if stoptest == 'TAA' or stoptest == 'TAG' or stoptest == 'TGA': stop_site = counter break countcodon = -1 countcodon = countcodon + 1 print "Start codon site: ", start_site print "Stop codon site: ", stop_site for i in range(alignment.get_alignment_length()): col = align.get_column(i) s = Set() # create a new set for c in range(len(col)): s.add(col[c]) # add each column element to the set if len(s) > 1: # multiple elements in s indicate a mismatch if i<start_site or i>stop_site: #To determine if intron or exon print "Exon: ", i, col; else: print "Intron: ", i, col;
output:
Start codon site: 60 Stop codon site: 1011 Exon: 3 AAT Intron: 658 AGG Intron: 802 C-A Intron: 803 C-C Intron: 804 G-G Intron: 805 A-A Intron: 806 G-G
Note: slightly modified apoe.fasta file to introduce a mutation before start codon.