Harvard:Biophysics 101/2007/Notebook:HRH/2007-3-13
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Link to assignment:
http://openwetware.org/wiki/Harvard:Biophysics_101/2007/03/13
Task 1
After running the NCBI Blast, I received the following results:
NT_030059.12 Homo sapiens chromosome 10 genomic contig, reference assembly NW_924884.1 Homo sapiens chromosome 10 genomic contig, alternate assembly (based on Celera assembly)
Additionally, the flanking proteins are:
- 3895 bp at 5' side: hypothetical protein
- 425 bp at 3' side: HtrA serine peptidase 1
There is a point mutation at bp 201 (A-T -> A-G).
The reading frame containing the ATG start codon has the mutation changing the amino acid from a CAG to CGG (Glutamine to Arginine). Glutamine is polar and neutral, and Arginine is also polar and strongly basic (they are both hydrophilic).
Laymen's terms: the mutation probably will not do any harm (i.e. don't worry).
Task 2
CAGCCGGGTGACCCAGGCCGAGGCCGGCAGAAGACAGCCTGATGCCTTGAAGACTTCCTCTTGCACTTTT GTTGGAGGGTGCTGGTTTGCTAAAAGCAGAGAGTATTTTTCTTTTTATTTATTTTGTTTTTAATTTTTTA ATTTTAGCTCCAGCTCAGTTGCCCAGACTGGAGAGCAGTGGCCAATCATAGCTTACTGCCTCCTGGAACT CCTGGCTCAATCGATCCTCCTGGATAAGCCTCCTCCGGGTACTATAGGTGTGCACCACCAAGCTCAGCTA ATTATTTTTTAGAGATGGTTGTCTCTATATGTTGCCCAGGCAGGCCTTGAACGCCTGAGCTCAAGTGATC CGCCTGCCTTGGCCTCCCAAAGTGCTGGGATTACAGGCGTGAGCCACTGCGTCCGGCTATTTTATTTATT TATAGAGACAGTGTCTCACTTTGTTGCCCAGGCTGGAGTGCAGTGGTACGATCATAGACCACTGCAGCCT CAAACTCCTGGGCTCAAGTGATCCTCCTGCCTCAGCCTCCTAGAGCAGCTGGGACTACAGATGCACCCCA CCATGCCTGACTAATTAAAAAAAAATTTTTTTTTAGAGATAGGGTCTTGCTATGTTGCCCTGGCTTAGTT TTCTTTATGTATGTTTTTTGGGGTTGGAGCTGTAGTGGAACCAGTCCCAGCCCCACTGGGCATCTGGGTC TGACCTTAGCTGCTCCACTAAGGGCTTAACAGGACACTGGCCACAGGCCTGCTCCCATCCAACTCCACTG GGGCCACAGGAGGGACCCTACCCAGGATGGAGTGAGGGCAGGCACAGACCAAACAGTGCAGGGTCGGGGG GTACTGTTCACTGGGACAAGAGATGAGGAAGATGCTGGATTTAAGTGGGCAATGAGGGTGCCTAGCAGAA GAGGGGACTCTTAACCTGGGTCTTGATGGATGAGTAGAAGTTCTCTGCATGGGAAGGGGTGGAGGTCATT TCAGGCAGAGGAGGAAGCATTAAACAGCATGGTGTATCTCAAGGAGAGCAGCTGGAGCATGAGTAAGGGG GGCTGGAGTAGGGGGCAGGGCATGATGTTGGGTCCTGTGGGCCTGGGTTAGCCCCAAGGCAGTCACTGGT CCTCCACTGACTGTGTGGTCCTCGCTTGGGCTCAGAGGGAAGGGGAGTGATATCCTGGGCTGGTGGGGTG GATAGCAGCCAACCCACCAAATGGTGGTCACGACCACTGCTGACAACAGTGTGCTGTGTGCTGGGCTGGG TGTCCACTCTGTCCTCCAGCATCTGAGGCTTGGGATCTCCCAGAAAGTCACACCAGGATGGGAGCCCAGG TCGTTTGAGCTGGGTGCTCTTGGCTGCTCACTTGCCTTCTTCCTGTCTAGGCCCCTTATAGGTTCACCTG GCACTGGGACTTGAAGTAGTTATGGCCTGAGGTCTCACTTACCTGGGCTGGCAGTTTTCTGAGTTCGTTC ATCTCTACCCAGCCCCCGTGGGGCCTTGCGCTCAGTGGCTGTGTGCAGAATGAACCAGTGGAGAGAAAGG GCTGCACGTAGCGCTCAGGATCCTGGGGTTGGGTTTGTGGCTGAGTGGACCAGGGGCTGACTGAGTGGAG CCCGTGGCTCTATCTCTCCCTGGGGTCCCACCTGGAAGCCCAAGGCCCTCAGAAAAGGGGTTTCCTTGCC TGTCATGGCCCTACCCTCCAAGCTGTGTCCTGGATCTTGGCAGTGTCCCCCTGAGCGCCCTGGCTCAGAC ATCTCCTGCCTGACCGTAGCTGAGCCACGTGCCTTTGTCCTTTATTGCAGCTTCATGGCGGCTGAAGAGA TGCATTGGCCTGTCCCTATGAAGGCCATTGGTGCCCAGAACCTGCTAACCATGCCTGGGGGCGTGGCCAA GGCTGGCTACCTGCACAAGAAGGGCGGTACCCAGCTGCAGCTGCTGAAATGTGAGTCCCTGGGGTGGTAG GGTGCACAGTAGGAGGGCATGGGGGCAGGGCTAGCCATGTAAGTAAGCATCCTCTCCAAGCCTCTGCTTC CTCACATGGTGTGCCCTCTGGATTTTCCCATTCCCTCTCTGGTGGCTGGCACCCCTGGAGAAGCAGAGGT TGACTTCCCTAGCCCCCAGGACTGTAGCAGGGTCCGGGGTGGTGCACAGTGCTGGCCAGCTGGCAAGGGG AGCTGCAGCCTGCAGCCAGGACCTGGGGACCACAGGTGGCCAGGTAGGTCTGGCCTCTGGTGCTGCCCTC ACTGAATCCGTGAATGTTCAGTGAGTTAGTTGGTCACTTCGCCCACAGTGAACCTATGTGTCAAGTCCTG GCTGGGTGCTGGACCTCTGGAGCTGCACAAGACCCAGGCTCTGGCCCCCAGGACAGCTGGCTGGGAGGAA GAAGCAGCCCTCAGGCTTGAGAGATACCCAAATGCTGAGATACTGAAGGCCAAGAGCATCAGGGCCTGAG CAGCAGGTGCTCCATGCCTTTGCAGTAGCAGTGCCCTCTGCCAGGGACTCCCTGGTCCTGGGCCGCCTGG TTGAGATGTCCAGGCTGGACTGATTGGTTCCTGTACGGGCACTGGTTGAGCTGTTCTCCTCTTTGCACAG ACAGCCTCCTTGGAGGAGCTGTAACTGGCTTGTCCCCCAGCCTGGGACAGGGCTGGGGCAGTGGAGCTAG GGTGGTTTCAGTGTAACTTTCCACCTAGGGGTGGCCCCACACCAGTGGCTCTCACTGGCAAACTCTCCGG GCTCCTGAGTCTCCCTCTTTTATTTTATTTTTTCGAGACAGGGTCTTGCTCTGTTGCCCAAGCTGGAGTG TAGTGGCGTGATCATGGCTCACTGCAGCCTCAACCTCCCAGGCTCAGGTGATCCCCTCAGCTGGGACTAC AGGCATGCCACTATGCCCTGTTAATTTTTTTTTAAATTTCTTGTAGAGATGGAATTTCATTATGTTGCCC AGGCTGGCCTGGAGCTCCTGAGCTCAAGCGATCCATCCGCCTTGGTCTCCCAAAGTGCTGGGATTACAGG TGTGAGCCACCGCATCCAGCCTATTTTTTATTTATTTTTGTTTTTTATTTATTTATTTTTTTGAGATGGA GTCTTGCTCTGTTGCCCAGGCTGGAGTGCAGTGACTTGATCTCAGCTCACTGTAACGTCCACCTCCTGGG TTCCAGCAATTCTCCTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGAGTGTGCCACCACGCCCAGCTA ATTTTTGTATTTTTAGTAGAGACAGCGTTTCACCATGTTGGTCAGCCTGGTCTCGAACTCCTGACTTCAA GTGATCCTGTCTTGGCCTCCTAAAGTTCTGGGATTACAGGCATGAGCCACCGCGCCTGGCCTATTTATTA TTTTTATGTCTCACTCTGTCACCCAGACTGCAGTGCAGTGGTGCGAACACAGCTCACTGCAGCCTCGACC TCCTGGGCTCAAATGATCCTCCCACCTCAGCCTCCCAAGTAGCTGGAACTACAGATGTATGCCACCGTGT CCGGCTAAGTTTTGTATTTTTTGTAGAGATGGGGTTTCACATGTTGCTCAGGCTCACCCCATCTTTTATT TTTAGAGACAGTCTCACTCTTGTCACCCAGGCTGGAGTGCAGTAGCACGATCTTGGCTCACTGCAACCTC TGACTCCCGGGTTCAAGCAATTCTCCTGCTTCAGCCTCCTGAGTAGCTGGGATCGCAGGCGTGAGCCACT GCACCCGGCCTTAGTCCCCCTCTTTTAGCTCTGGGGTAGTGGGAGCCTCCAACAGCCCTGCAGCACCCAG CAAGTCAGCTGTGGCCTCCCTGGGCCTCTGGTCCTGGCTCTGCCTTGCCTCATGCTGGCTCCACACAGCC CCCCAAATCTACCTCTTCTTTGTGGGCAGCCAGGCCTGCCCTCGGGTGGCACTCTGGGGCTAGCCAGTGT GCCTACAATTTTTTCCGTATTTGGCCCCAGAGTGGGCTCCTGGGGAGGGGGCAGCATGCCAGCCTTGCTC CCTGCTCTTCCATTTGGTAAGGCTTAGAGGCAGGAGGCCTGGCCCCAAGTTGTCCTGTGGAGGGTCCTCA CAGTGGGTCTTGGCAGCTGCCCCTCCAGGCTCACCTTCCTGCCCTTGCCACCTCCCACAGGGCCCCTGCG CTTTGTCATCATCCACAAACGCTGCGTCTACTACTTCAAGAGTAGCACCTCTGCCTCCCCGCAGGGCGCC TTCTCCCTGAGTGGCTATAACCGGTAAGTGCCCGACCTGCCTGCTGACCTCGGGCCCCCACAGCCAGCGG GTCCCTCCCAGCAGAGCCCCAGCTGGGCCTGCCCCTTATTCCCGCCCAAGGAAAGTGCTTTCCCGAAGCA GCCTTCGTGCCCATCCCCTGTCCTCCCCGCCCAGCCTCCACAGTGCCTCGCCCTCTCCGCGTGTCCTGCC CTCAGGCGCAGGACCTTGGGGGTGCCAGGGTCTTCCTGTGGCTTTGTGCACAAGTCCCAGAGGCCTGGAC AGCCTGCTCTCCAGAGTCTCCACTCGGCCACCACCCAGTGACTTCTGCATCTCTCCTCCCTCCCCACCTT GCCCCTTCACCCACCATGACCTCTTTCCAGGCAGCCCAGACCCCAGCCAGCCCAGGCCCTGTCCCAAACA GAGGCTGGGCCCTAGGATTCCCTGCTCCAGGGTCCTCCCAGGCCGGGATCAGTTTCCAGCTTCACGCATG TCCTGGGCTGGGCTCTATCCAGTGATGGGCCATGAGCTGCCCTGGAGGGCCTTCTGCACTAACAGGCAGG TGGGCGGTGGCCAGGTGGCATGTGGGTGGGGGAGTGCACTGGCACAGCCCATGGCCTGGGGCCCACACAA AGGGTTAATGCTGGCACCCAGCTCTGGTTCTGGTTGCTATCCCATCGGAGCCCTGCACCCCACAGCATTT ATCTATGCCTCTTACCACCCCTTAAGGGCCCAGCCCCTCCTGCCTATGAGGAACCAAGGGTCACATGGTA TCAGTAGTGGAACTGGGACCTTTGGAGTGACTCTAGGCCCCGGCCTGCACCCTGGCCACAGGCCTGGTAG GGCAGCCTTGCCAGAGCACCCAGCCTGGTGCCAGGCCCCCATGCTGGCACAGAGCTCCGGGGTCCCCTGG GGAAGGTCGTTCCCTTTCTGCAGACAGACACAGCGCCTTCCGGCCTCCAGCATGTGCCAGCCATGCCATG AACCCTGGGCCCAGTGGCAGATGCAATCATGCCCCAGGGAATTGTCTAGCCCATCAGGGAATGCTGGGCC TGAGGAGTTTTGGCCAGGCTCTTATGGGCCGTATAAGTGCACCTGCCCCGGTGCCTGGCTGGCAGGGCTC CCCACGTGATAAACTCAGCGCAAACCCCACATCCTGGCTGTGTGAGTGGGCCGCAGAGCAGGCACGAGTC ATTCCCTGAAATGCACTCAGGGCTGCTGCTGGGCCTCAGGGGCACGGAGCCCTGTGGGGTACCCCAGGGA GGCCAAGCAGGCCTGGGCAAATGGGGCCAGAGGTGGGAGGGTGGCGTCCATGTAAAGTGGCATTGGCGGG CATCGAGGCAGGTTTGTCCTTGTCTCCTGTGCACTGCTGCTCCCACGAAGCCCACCCAGCACCCCTCTTC CATGCCCCTCCATGCCCCCTTACGCCTGCCTGGGCATGAAGCTCACTACTGGGAGCATCAGCAGCCAGGG CCCACCCAGATCTGCCCTCTTCCGTTGGGGCGTGGTGCTGGTGCCGAGACGTGTTCACAGGGGGGTGCTG TGGGAAGGCCATCCCTATAGCTGTGAACCAGGCCGTGACCCCTGGCGCTGTGCCCCCAGGGTGATGCGGG CGGCTGAGGAGACCACGTCCAACAACGTTTTCCCCTTCAAGATCATCCACATCAGCAAGAAGCACCGCAC GTGGTTCTTCTCGGCCTCCTCCGAGGAGGAGCGCAAGGTGACTGGGGGTCCGAGGACGAGTGCAAGGTGA CTGGGGGTGTGGGCCTGCAGGCACCAGGCTGGACCTGCCTTGGGGGCTCGCTGGTCCTGGGGCGCTGGCC TCTCAGCCCCGGGCAAAGAGAAGGTGTGGCTGGGACACAGGCAGCTGGGTGGGTGCTGCCCCAGCTCCAT CCCCATGCCCAGAGCCTGGTGCCAGCGCCCACACAAGGAACATGCTGTCCTGGGAGGTGCCCCTGTGGGC TGAGGGGAGCCACACAGCCATGTTGTATCCAGCCGGGTCGCCTCCTCTGACCTTGGGAGTCACAGCAGAG CAGGCAGGGCAGTGAAGGTGGAGGGGTGCGGTGGGGCCCACCCTGGTGGCACCGTGCCCACCACAGCCCC GCTGACCTGCAGAGCTGGATGGCCTTGCTGCGCAGGGAGATTGGCCACTTCCACGAAAAGAAAGACCTGC CCTTGGACACCAGGTGAGCCCGGGCCCAGGGCATACCGGGCAGTGAGGGTCCCTGGGGCGCCTGGGCCTG ACCCGGGTGTCCGCCTCCCAGCCCCGGGCTTGGCATAGAATTCCGTCCTTAAAGGTTTCCTGGAGGTGCC AGCTGGGCTGCGTCTCTCTGCTCCTGTCTACAGATAAACACAGATACAGGACAAGCACAGATGTTCACAC ACAGACGAGCGACACGCGGACACGCACACACAAACACAAGCAACACGCGGACATGCACACACACAGAGCA ACACGTGGACATGCACACACACACAGAGCAACACGTGGACATGCACACACACACAGCAACACGTGGACAT GCACACACACACAGCAACACGCAGACATGCACACACAAACACAGAGCAACACACAGACATGCACACACAC ACAGAGCATGCACAGGTGTGCATGCATCTCTCTGCTCCTGTCTACCTCCAGCCCCATCTGTCACATCTGT TTCTGTGTTGCCGCCATGGGTAGTTCCTGTTAGGGGATCATGTGTGTTTATACGCCTGCCACCAGGGAGG CCTGGCATGGCAGCAGCTGGGCACTTTGGGTTCCCAGCCCCCTACGCCCACCCTGGCTCACTCAGAGTGA GACCAGAATGATTCTCCTGGCGGGGCGCCACTGGGCAGTCTGAGCCTTGGCAGCCTCCCCCGTGAGCTGG CCCGACGTGCAGCAGATACTCTCTGGATTGGGACTCCCTGGCCCGAGACGATGTGGAGGGAACAAACCTT CCTGTCCTTTCACAGGTGGGGACAGGAGGCCCAGGGAGGGCTGGGCACAGCCAAGGCTGCCCTGCAGGTC CCTGCCTGACTCCGGGCTCCCGTCCCCTGGTCCAAGCTGTTCAGCCTCGGGCCCAGCCCAGAGTCGCCCA AGCCCTCTTGAGGCAGGAGCAGGTGGAGACGTGGGCACAGTCAGGGCGGCCATCCTTGTGGGCCAGAAGG GGCACCTAGTGGAGGCACCACAGCCTCCAGTTCTTCTGTCTGTCCTTGTAACTGTGTGTGCATGTCCGCG TGTTGCTCTGTGTGTGTGCATGTCCGCGTGTTGCTCTGTGTGTGTGTGTGTGCATGTCCACGTGTTGCTC TGTGTTTGTGTGTGCATGTCTGCGTGTTGCTCTGTGTTTGTGTGTGCATGTCCGCGTGTTGCTCTGTGTG TGTGCATGTCCACGTGTTGCTCTGTGTTTGTGTGTGCATGTCCGCGTGTTGCTCTGTGTGTGTGCATGTC CGCGTGTTGCTGTTTGTGTGTGCATGTCTGCGTGTTGCTCTGTGTGTGTGTGCATGTCCGCGTGTTGCTC TGTGTGTGTGTGTGTGCATGTCTGCATGTTGCTCTGTGTGTGTGTGCATGTCTGTGTGTTGCTCTGTGTT TGTGTGTGCATGTCTGCGTGTTGCTCTGTGTGTGTGTGCATGTCCACGTGTTGCTCTGTGTGTGTGCATG TCCTCATGTTGCTCTGTGTGTGTGTGCATGTCCGCATGTTGCTCTGTGTGTGTGCATGTCCGCGTGTTGC TTGTGTTTGTGTGTGCGTGTCCGTGTGTCGCTCGTCTGTGTGTGAACATGTGTGCTTGTCCTGTATCTGT GTTTATCTGTATACTTCCATGTCTGTGTGACAGAGTCCTTGTGTCTGTGTGTCTACATGTCTGCGCGTGT CCCTGTGTCTTTGTGTATATATATCCATGCCTGTGTGCCTGTGTTCCTGCGTGTGCTTGTGTGTGCACGT GTGCATTTGTGTGTTTGTCAGAGTATGTGTGCATGTGTGTGTCTGTCAGCGTATCCATGTGTGCATGTGT GTGTCTGTCAGCGTATCCGTGTGTGCATGTGTGTGTCTGTCAGCTTAACCATGTGTGCATGTGTTTGTCA GTGTATCCGTGTGTGCATCTGTGTATCTGTCCATGTATCCGCGTGTGCCTGTGTGTACCTTTGTGTGAGC ATCAAGGGACCTCCCAGGCCTGGTGCTCACCGTCCGCCCCAACGCACCCTGCATTGCAGCGACTCCAGCT CGGACACAGACAGCTTCTACGGCGCAGTTGAGCGGCCTGTGGATATCAGCCTTTCCCCGTACCCCACGGA CAATGAAGGTGAGGTCTTTCTCCGCATCCACTGCCCGTTCGCCTCTCCCCACCTGGCCTCCTCTTGGCCG CTGTGGAGGAGAGGGAGGTGTGTTCGGCTGTGCTCCTTGCTCTGGCCCTTTGGCAGTGCGCAGTAGCATC CCTGGGCTCTGGCCTTCAGCGGCAGGGTCTGGACTCACAGTCCTCCCAGCAGGTGCTTGCCCCTTGCCTC CTGGACTCAGCCCCATGCATGGAGCATTGCTCGGAGACTGGGCCTGGGCCGGTTTGGCTCTCACCACCCC CCTCTCCCCATGCAGACTATGAGCACGACGATGAGGATGACTCCTACCTGGAGCCTGACTCCCCGGAGCC CGGAAGGCTTGAGGGTAGGTGGGGCGGGTGGGCCCGGGGAGCTCTGGGTGTGTAGGAAGCAGGGGCTGGG ACCGGGAGCAGAGCCCCTCCCAGGCTGGGGATGCTGGCCCAGGTACCGCTCTGGGTGGCTTCCGCTTCCC TGCTGTGTCCCAAGTATTATGAGGATTGGAGCAGAAGAGGTGTAGCTGCTGCCACCTCTGCTCATTTCCT CCTCCCACCTCCCAGGAGGAGGGACAGGCACATGGCGATAAGTGGCAGGTGGAGCCGGGGTGGCCCTGGG GGTTAAGCATGGGGAAGGAAGGACGCTGAGGAGCGCGCAGGCTCAGGGCAGGTCCATGTCCCCATGGCTG CCAGCTTGTGTGGACACTGCTGCAGGCCAGGTCGTTGTCCCACCCCACCCCATCCCATCCCAGTGTCCAC CTACCTCCTGCAGCACATGGCCTAGTCAAAGAGACAGGCTGATACAGGTGGTCACACGTGGATGACGGAA GCACGAGCTGGGTGGGGAGGACGTGTCTCTGTCCTCCTCTGTCCCCTCCCTCCCTTCCTCCCTCCCTATT GTCTCTCCAGCCCCCTCATCTTGTCAATTTCCTCATGTCTGGGGCTGATCCTTGATCCTTCCATTTTCCC TGCGTCCGCCCTGCCTCCTTGTTTTCCAGCCTCCTGTTCTCACTGACTTGGCCTCTGCTGACCCCAGCCC CTGACCCCGTATCCTCTCTGCCTCCCATCCCCTCCCACCTTCAGGCTCCCGGGTCCAGCCCCTCTTCTCC TGCCGCTCCCTCCCTCCTTCCCTCTGGGGTCCCAGTGCCAACAGTCCTTCAGCCCACCAGGGTCTGCGGG CCGCTTGCCTCAGTTTCCTTCCATGCAGAGCTCTTGTCTCACAGTGTTCACCATGCCTGTCCCTTCGCCC ACACACTACTGCCTTCCGTCTTAGCCTGGCTGGCCAAGCCTTGGTGAACTCAGACTCCGTCTGCCCAGCC CTGAGCTGCAGCCCAGTGAGGCTAGAGATGGGCCTGGGGAGCCACCAGTGCTCCTGCATGCTACTGCTCC ACTCACCCTCCCTCTGTACCCCCTTCATCCTCTCCCCGAGACATTTCTCGTCCAGGACGCCTGTGGCCTC CCCATTCCCAGTTCCAGCGCTCAGTCCTGAGCCACACCAGCCTGGCGCCTGCCCTGTTCCTTGCCAGCCC GTCCTCCAGGCGCCCCGTGCTCCCAGGGTCCTCTTTCTCCTCAGCTCCTGTGCTGGCCCCATGAGGGATA CCCGGGACTCAGACCTCACTCCTCTCCCGAATTCCCTGGGTGACAGCCTGGGTCATCTGCAGGCTGCCCG CTCCGAGTCATGTCCAGCCTGGATTTTTGAACTCTGCACACATTCCCTGACCCCGCATCCCCTGGCATCT CCCAGCTTTCTGGATATGTTCTGAGCAAACACGGGCCTTCACCTTCCTCCCAGCTGCTCCCCTCCCCTGT CCTTGGGAATTCCGTCCTCCCTCTCGCTCCAGCCGAGCCCTGAGATCCTTCTTGACTTCTCTTCCTTTCT CTTCCTTCCACCTCTGGGAACCTGATGGGCTCCTCAGGTGTCCTCTAGGCTTCAGGAAGCGTCCACAGGC TGCCATAGGGAGGATGAATGGGGAAGGCTGGGGGTGGTGGGTGGTCTGGGACCCTGGGGAAGGCAGGATG GGAGTGCTGGGTGCTGGGCTGCTGGGTGGGCAGGCTGTGGGGTGGGCCTACCATGGGTTGCACTCCTGGT TGGCCTGGCTGACCACTGCCAGCAGAGGATAGTGTTGGCCCAGTCTCTGTCAGGGTCCAACCCGGGTCTC TTTGCTCTGCAGATGCCCTGATGCACCCACCGGCTTACCCACCACCCCCAGTGCCCACGCCCAGGAAGCC AGCCTTCTCTGACATGCCCCGGGCCCACTCCTTTACCTCCAAGGGCCCCGGTCCCCTACTGCCACCCCCG CCCCCTAAGCACGGCCTCCCAGATGTTGGCCTGGCGGCTGAGGACTCCAAGAGGGACCCACTGTGCCCGA GGCGGGCTGAGCCTTGCCCCAGGGTACCTGCTACCCCCCGAAGGATGAGCGATCCCCCTCTGAGCACCAT GCCCACCGCACCCGGCCTCCGGAAACCCCCTTGCTTCCGGGAGAGTGCCAGCCCCAGCCCGGAGCCCTGG ACCCCTGGCCACGGGGCCTGCTCCACTTCCAGTGCTGCCATCATGGCCACTGCCACCTCCAGAAACTGTG ACAAACTCAAGTCCTTCCACCTGTCCCCCCGAGGACCACCCACATCTGAGCCCCCACCTGTGCCAGCCAA CAAGCCCAAGTTCCTGAAGATAGCTGAAGAGGACCCCCCAAGGGAGGCAGCCATGCCCGGACTCTTTGTG CCCCCCGTGGCTCCCCGGCCTCCTGCGCTGAAGCTGCCAGTGCCTGAGGCCATGGCGCGGCCCGCAGTCC TGCCCAGGCCAGAGAAGCCGCAGCTCCCGCACCTCCAGTGAGTTTGTGTGGCGGCTGCAAGCCCTGCCTC CAGCTACAGGGACCCTGGCCTGGCCTCTGACGGGCACTCTGCCCACCTCGCTCCCCTGGCACTCTTAGCC CACGACGGGGTACCAGGTGCAAGGGTAGGAAGGTTCCCGGTTGCCTCCAGTCCGCCTTGGTGGAACGTGG AGCATGTCCATGACTCAGCCCTGAAGCTTGCTGGAAATGCTTTGTTTTTTTTTCTTTTTGAGACAGGGTC TCACGCTGGCAACCAAGCTGGAGTGCAGTGGTGCGATCTCAGCTCACTGCAACCTCCACCTCCGGGGTTC AGGTGATTCTCCTGCCTCAGCCTCCCAAGTAGCTGGGACTACAGGCACCTGCCAACACGCCCGGCTAATT TTTGTATTTTTAGTAGAGACAGGGGTTTCACTATGTTGGCCAGGTTGGTCTTGAACTCCTGGTCTCAAGT GATCCGCCTGCCTCGGCCTCTCAAAGTGTAGAGATTACGGGCGTGAGCCACCGCGTCCTGCTGAAATGCT TTCTGCTTTATTTCTGGGAAGTGCCAGGAGACCAGTTTGTCCCCCACTCCCACAGAGTTACGCCAATGGC TGTGGTCAAAGGCATCTGGGACCCCAGGTGGGCTTCCTGCCTTGCCTGGCAGGCAAAGGGTCCAGGAACT GGAGGGGCAGGTTTCGCATGGGTGTGCTGGCCGGCACGCGCACCCCCGAATGCCCTTGCCCACGGTGCCC TTCTCCCAGGGCTCTGCGAACACCTCTGAGATGCCTGCTGCCTGTGTGGAAGTGCCTCTGAGCAGCCCTT GTCACACTCGTGGTTCTAGTTCACCAAGGGCTGCAGAGATGCAGATGTTCGGAAGTCACGTTTTTCCATG ACTGTGGGGATAGCGGTTCCAGGGCTCAGCCTCACCCACAGGCTGGCCTCCCATGCTGGCGTGGCCCGTT AATCCTCCCTAGAGCCTTTGAGGCTTTGCTGAGCAGGACCGGCTAGCCACACAGGGAGGCGTGCAGGGAC GCCATGGGCGTCCTTTGGGGTCACACTGCATTGTTCATCCAGTTCACGTCCACTCCCCTGTGGCTCTGAG TGGGCTGTGATTTGGCCATGACCGGACCTCCCCTCCCCACGAGCATCCAGAGCACTTGTTGACGGTGTGA TGACACTGAGCTTGGGAACCCTGGACTCCCTTGCCAGCATCCATGGCAGCCCTGACCCCTGACTCCGGTG TCGGGCGATTCCTGCTGTCCCAGGGCAGTCAGCACGAGCCCTACCCGGATCTCTGTTGTCCTGAGCTTTT TAGGGTCACAGGGGCCATAGCAGGCAGCTTGCCGTCCTCACACAGAGGGTGGAGTGGGGAGGGGAGCAGA GGGTGGCCGCCCCGTGTCTGACAGTGAAATGGTCCTGCCTTCCTCTCCCTGCCCCTCCAGGCGATCACCC CCCGATGGGCAGAGTTTCAGGAGCTTCTCCTTTGAAAAGCCCCGGCAACCCTCACAGGCTGACACTGGCG GGGACGACTCGGACGAGGACTATGAGAAGGCAAGGCTGAGCGGCAAGCCTGGGTCCCAGTGGCCAGTAGG TGGACAGGTGGTGGGAAAGCCATAGGCCAGGGCGGCCCCTCACAGACCGTCCTGAGCAAGGACCCCCCGA GAACCCGGGAGCCTAGGGGGACACAGCACCATGTAAAGCCCCGGATCCCGGCACCGGGTGGCCACCGTCC GGGGAGTGGTGGTGCGGGTGGATCACTCCGACGTTGGCACTGACACCGTCAGCCTCTTGCAGGTGCCACT GCCCAACTCGGTCTTCGTCAACACCACGGAGTCCTGCGAAGTGGAAAGGTCAGCACAAAGCCCTGTGTGT GCTGGGTCCTCCGCCATGCCCGGCTTCCTGCTTCTGTGTCCCTCTCACTAGCTTCCGTGTTGGGGAGTTG CTGGCACAAGTTCATGGCCCTGCGTGCAGCAGAAACCAGAGGAGTGGACCTCCCTGCTCTGTCCCATGCC CAGCTGGCACCCTGGCTGGCCAGGGCTCTGCTGGGCTGCTTCTGTCAGCCTCACGGCAGCCCGACGTGCT CAGCTCCTGAGACCTACAACAGCGAGAGGACAGAAAGCCAGGCTTGGGAGCGGGGCGGGAAGGTCCGTGT GAAAGCTGCCCGAGGAGGACTCACCCGCTAATATGACTGTCTTATTTTAGGTTGTTCAAGGCTACAAGCC CCCGGGGAGAGCCCCAGGATGGACTCTACTGCATCCGGAACTCCTCTACCAAGTCGGGGAAGGTAGGCGC CAGGGGAAGATGCCCCAGGGCCCCTCTGGCTCTCCACACACCCAGGCTGTGGGTGGGCCCAGGACTCCAC AGTTCCTAAACCACTCCCCTTGTGGACTCTTACTTGGTGTCTCAGCTGAATTCTCTTCCCAGCAGCACCC CCCAATCTATTCCCTAATAGATCTCCATCTGCAAATTGGGGGCCATGATGGCCACCTCCCAGTGGGGGCC AGGACTCGGGTGGGGTAGGGCTCATGCTCTGGGGTGGCGGAGGCCACATGGGGCAGGGGAACGCCAGGTC TGCCCGTGTCCCTCCGAGCGTGAAGTTGTGACCTCACTGAGCCATGCTCTCCCGGGTGTGTCGGAGTGGT TATTGGTGGTTATGAGCTGTTGCTCATCAGAGGCTCTCTGAGGGCTGCCAGTGTCGTCCCCGCTGTCCTT GTGCAGGTGGTCTGGAGTCCCTCCCCGCCCCCCGAGGGCCACAGGACCCAGCCTTGATGGTTCTGCCCCC CTATCCCCAGCCCATGGTCTCCCGAGGCTGGTCCCGCTGTTTCTCAGGGAGCCGTCAGCCTCCCGCTTCC GTCCTGTAGGTCCTGGTTGTGTGGGACGAAACCTCTAACAAAGTGAGGAACTATCGCATTTTTGAGAAGG TGAGAGGGCTCTGAGTGGGACGGGGACCCTGGCCGCATGGCCTGGCAAGGGGCAGGGCAGAATCTCCCTG ATGAGGCATAGGCAGCGGGTAGACTGAGACTGGCACCTCCAGGATACCGCCCTCCCCTTCCCCTCCACCA TCGCTCACCCCCCACCCCTCCTGCTCAGCCTCCCTCCTCTCGTGGCCTACCTTTGTCCTCCACTGACCCT AGTGGGGATGGGCGGTCAGCCATAGACCCTGGGTTGCTTGTCTTGTCTTTTTCTTTTTGCGGGGACAGGG GTCTCACTGTCTTTCTCAGGCTGGTTTCAAATTCTGGGGCTCAAGCAATCCTCCCACCTCGGCCTCCCAC AGTGCTGGGATTACAGGCGTGGGCCACCGTGCCTGGCCTAGGTTCATTTCCTGACCTTGTCTGAAGTGCT CTGGGTGCAGGCTCCTGGACATGGAGGACGGAGGGGAAGTGAGGTGGGAACATGGAGAGCACAGGCCTGA TGCGGAGGCCACCTTGGGGGCACCACCGACAGCCAGGGGCCAGCCTGGTGATGCCGCTGTTGATGCTGCT GCCTTGTTTTACAGACGGGGAGACTGAGGCCTAGAGCCGCAGAGTGGCCTGGCCCTGCTGACGCTCCCCC TTCTCTTCCCCCACAGGACTCTAAGTTCTACCTGGAGGGCGAGGTCCTGTTTGTGAGTGTGGGCAGCATG GTGGAGCACTACCACACCCACGTGCTGCCCAGCCACCAGAGCCTGCTGCTGCGGCACCCCTACGGCTACA CTGGGCCTAGGTGATGGCAGTCCATGTGGCTGCCAGGCCAAGGCAGTCACAGGGGCCCTGACCCCAGGCC ACACAGACGGACATGGGCCCACATGGGAGGGTGAGCAGGAGCAAGGCTGTGCTTGCCTAGGGCCTCTGTG ATGGACATCTCGTAGGACCCAGCCAGTCTCATCCAGCAGGTTGGGTTCTAGGGCTGAACCAGGCGCCAGG CTCCAGAGGACGAAGGGACTCTGTTGCCCCACACTAACTTGCCCTGTCCCAATCCCAGAAACCCAGGACC AAGCTGTGCCTGGGCTCCAAGGACAGGAACACTGGTCCCCCCATCACACTCACCCCTAAGTGGGCTGGGA GCCAGGCAGGGCCAGGGCAGCTGGGTGGGGGCCGGGGCTGGCCCTGGGACCCCCAGGAACGCTAAGACAC AGGCTCCAGTAGGGGCTGTTGCCTCCAATAAAGCAGCAGTGAGCTTTGCCTTGGTGGCTGGGGCTTGATT GGGAAGGAGGGGATTACCAGCTTACTGGGTGCCCATGCTGATGTCTAAGTGGTGACCGCAGCAGTACCCG GGAACCCCAACAGTTGGTTGTCTTGTCTTCCAGGGTGCAGGTCACTGAGTGACTTCCCCAGGGTGCACAG CGAGTAACAGATCAGGACCCAAACTTGGGCAGTCTGGGCTGGGAGCCCACACCCCACTCACCAGTTCTGC TGCCTCAGGTCAGGCCAGGGCAGTGCTGCTGCAGAGCTAGAAGGCCCTGCAGCTACAGCTGCTTCATTCC CTGCATTAGTGCCTGGTTACTGGGTACCTCCTGAGTGGCTGTCCCCGTTCCAGAACTTGCATACACTGAG CGGGCTACAGAGCTAGAAGGCCCTGCAGCTACAGCTGCTTCATTCCCTGCATTAGCGAGCAGTTATTGGG TACCTCCTGCATGCCTGGTCCCATTCCAGACAGGGGCCTCTGGCCTGGCTGAGTTCACAGCCCAGTCTGG GGACAGCTGGGTATGAGGTGCTTACGGCACAGTGTCCAGGGCAGCTGGGTGTGCAGGGACTGGGGGCTCC CGGAAGATTTTTTGGAGGAAGTAACAGCTACGATGGGATGGGAACAGTGGACCCTAAGCAGGCCAAGGGT GCGTAGGGACGGTGGTACCCAGATGCCCAAGTCTTCCAGGCAATACCTGGCTCAGGCCCAGCCCCAATCC ATCCCCTTACTTTCTGCCATGGAGTTCCAGCAGGTCACTCTCCCTGGCACACCTTCCAGGCTGGATTTTT AATGAAACAGACTCAGGGAGGTAGGGGCTGGCAGGGACCCTAGAATCCTTGTGATTTTTCTTAGCACCTT ATGTCAGGGAAACCTAAACTGAGGTCAGCACTTGGGCCCACTGACAGTGACTGACTGGGGGAGAAGGTCC TGCAGCCCCCTTCCCCTGGGTGTGTTCTGGGGACCTGTGGTTTGCTGGCGGAAACAAGTGATGAGGCTGG TTAGCGGATGTGGGAGGCTGTGACCCCAGGGGGCCATAGGGTGCGGTGGAACTGCAGGCCCTGCAGATGA CGGCAGCCAGCTGCTTCCAGGAACCAGGTGTCCAAGGCCACCTCTGCAGGGGTTTCCTCTTCAGCCTGCC TGGGGTGAGAGGTCAGTGCACCACAGCCGAGGCTGGAGCACAGGGAGCTTCTGTTGTTCTGATCTATCTC TGGAAAACCAGCCATTCCTCCTCCCTGCAGTCAGAATTCTTTGCCCTGTCTGACCTGAACTTGCTTAGGG AGTCATGCCACTCCCCACTGTGGCCATAGTTTCTCTTCCTGTAAAATTTTATTATTTTAGTTTTTTGTTT TTGAGATGTAGTCTCACCCTGTCGCCCAGGCTGGAGTGCAATGCCGTGATCTCCGCTCACTGCCACCTCC GCCTCTCTAGTTCAAGCGATTTTCCTGCCTCAGCCTCCCGAGTAGCTGGGATTCCAGGCGCCCGCCACCA CGCCTGGCTAATTTTTTGTATTTTTAGTAGAGACGGGATTTTATCATGTTGGCCAGGCTGGTCTCGAACT CCTGACCTCAGGTGATCTGCCCACCTTGGCCTCCCAAAGTGCTGGGATTACAGGCATGAGCCACTGTGCC TGGCCCCTTCCTGTAAAATTTTTAAATGGAGAATTGGGTGCGAGATGTGGTTTCCAGCCTGGTGCCTGGG GTGCTGAGCTAGTGAGTGGTGCAGTCCAGGACACCTTTGCTTTATGTCACTTACACGGTCACCTGGAGCC GGCTCAAGTGGCTAAAGCATCCTGGGGCCCAGAGCCAGGTGATAGGTCCCTCTGGCCAACTGGACAGTTG AGGCCTGTGGTTACCCGAAGCCCAGCTGGGGCCCTGGTCCAGCCTCGCCTCCCAGACTCTGCACCTGCTA GCACAGCTGTCCACGTCTGTGTGAGCTGCTCTAGGCCGAGGGCCTCAGTTTCAAGAGTGTGTTGGGGTGG GATGGGGCAGGCCGTGGTCCTCCAGCATGAAGAAGGAGCCATGAGGAGTTCCCATGACCTCCCGAGACTT GCCATAAGTGTTCTAGTCCACATATAAGGGTAGGGTTGGGATTACCATTTACTGACCACATCTGTGAGGT GCCGAGCTGGGTGCTTGACATCATTTGCTTGGAGAAGCAGCTGCTAGTAGACCCATTTTACAGGTGAGAG AACCAAGTCTCACAGAGGCCTGGGTTCAAGTCCCACCTCTGCCACTAACTGGCATGTGACCCTATCTATC CTTCACTGCTCTGAGCCTAGACCCTGGCCCCTGCCTGGCTCCCTGCCAGGCTCCCTGCCACCCCTCACGA CCTCTGATGGTCGTTGTGGGGGTCTCTTGCCTGGCTCCCAGGGCTAGGGTTAGGGCTCTGGAGGTGCTTT CACTCAACCAAGGGGGCCACAGCACTGGGGAGTGAAACTGCCCCGCCTCACCCTGCGTTGCCCTCTGGGT CTGTGAGGGTGGGCTGGCAGGAGGCCTAGGCCTTGCCCTAGGGGCAGTCCTGCTTCCTCATTTTATAGAT AGGGAAACTGAGGCTTTGGGAGGACTCACTGACATACCTACCTTCAAGATGAGTTCAGGTGGGCTCAGTT CTGGGGCTTGGGAAAAGGGCCCCAGTGGCTTTGGGAAGCACCCCCAGCCCAGGGTGAAACATGCTTCTTC TCTTCCTGTGGTTCCATCCGAAGGATTGTGGTGAGCCCCGTGCCTTCAGTTAATAAAGATTTGTATTGTG AAAAGATTTTTTCTTTTTTTTTTGGGACACAGTCTCACTCTGTCGCCCAGGCTAGAGTGGATTGGCGTGA TCTCGGCTCAATGCAAATCTCCAGGGTTCAATCGATTCTCCTGCCTCACCCTCCCATGTAGCTGGGATTA CAGCTGCCTGCCAAATTTTTGTATTTTTAGTGGAACCGGGGTTTCACCATGTTGGCCAGGCTGGTCTTGA ACTCCTGACCTCAACTGATCCGCCCACCTTGGCCTCCCAAGTGCTGGGATTACAGGCGCGAGCCACGGCG CCCAGCCTTGAAAAGATGTTTTTAGAACCAGAAGAAACCTCGGTTCCCACTGATCCTTCTGGGCCACGTT GTGCGGAGCTCCCCTGCTGGTTGGGGCTCAGCGCAGCCCCAGGGAGGTGCTTCCTGCACCTCAGGATGGG CGAGGGTGGGCATTGGGGGAGAGGGGGACCTGGGACCTGCGGCTTAGTTCCCTGAGGCAGGCAGGGCTTA TTGGGGCCATTTCATAGAAAGGCAGATTGAAGCTCAGCAGGGAAGAGGCTTTTGAGGGTGATCCAGGCGC TGGAGGGATGGCCTAGGACACCAGGGTCACACCAGGAACATGGGAGGGCCGTGCTTGTCTCTAGACGAGG GGAATGGGGGAAGGGCCACAACCTCTGTTTCTGTGACCCAGCAGCATCAAGCCCCTCGCTGGGCACCTCG CACACACCCCCTGCCTTATCTCTGCCTGCACGCCCTGTTCCCTCCACCTAGACTGCCTGCTGAGGGGGCA GTGCCAGGAGGTTGCCTGTCCTTGGGGAAGAGGGGCAGTGACCCTGTGAAGATGCTTGACAGACAACCCC CACCACCTCAGAAGTGTGTGTGAGTGGTGAACCCTTTTAAGCCATCTTCCAACCATTCTCACTGGAGGGA GATTTGATGGGTACAGAGCAGACCCCTACCTGTCTACCCTCCTTCGGACCCCTAGGAAGCTTCGCAGGCC TTCCAGGCTGCCAGACAGCTGCCCTGGCGTTGCCGTCTGCTTCTTCCCTGGCCCCACTCTGAGGGGCTCA GAGCTGAGGCAGAATCCCTTTTTCATTCATTTCCTGCAGAATAAAACAACATACAGAAAAGTGAATAAAA CATAAATGCACAACCTAACACACTGTTAGGAAGTGAACGATCTGCAACCACCATCAGGAAATAGTTTTGC CAGCACCCAAGTGCCCTCCCCTCACAGTGTCACTTCCGGCCTCTCTGCCCTGGCTTATGTGAGTCTTGTG TTCTTGTTTTTCTAAAAAGTCTTCAGCACCCAATTATGCAGGCATTGCAGTATTTTCCTGTTTCTGTGCT TTATCCCCTTGAATCATACAGATGCAAATTCTGGCAGCTGGCTTCTTTGGCTCGTTATTATGTCTGTGAG ATTTATTCATGTTGCTGTGCGTAGTATAGTTTGTGCATGTTCATTGCTAAAAACTTCCATTGTTTGGCTG TATCGTAGTTCACAGATTCATTTCACTGTCAGTCAAGCTTGTCCAATGCATGCAGCCCAGGATGCCTTTG AATGTGGCCCAACACAAATTTGTAAACTTTCTTAAAACATTATAAAGATTTTTGTTTGCGATTTTTTTTT TTAGCTCATCAGCTATAGTTAGTGGTAGTGTATTTTATGCGTGACCCGAGACAGTTCTTCCGGTATGGTC CATGGAAGCCAAAAGATTGGACATGCCTGCTGTAGATGGACAGTTGGTTTGTTTCTAGTTTGGGGTAACT ACACACAATGCTGCTAGCAACAGTTTTGTCCATGTCTCTGATGCACGTGTGTTTTTTGCAAATGGTGCAC AAATTTTTCTAGGGTTTGTACTCAGGAGTCTGACTCCTGGGTTCTAGGGTATGAAGATCTTTCTAAATAT TGTTCTAGTTTACGTGCCCACCAGCAGTAAAACAGAATTCCCTTGCCTTCCCATCCTTGGCAGACATTTC ACTTTTGCCAGTCTGGTGGGGTGTATAGTTATGGCCTTAATTTGCATTTAGCTAATTACCAAGGAGATTG AGCATATTTTTATGTTTTTATTAACCATTTTGATTTTGTCTCCTGTGAAGTGTCTATCATCTTTTGCCCA TTTTTTAACTTGTTGTCTTTTTCTTTTTCTTTTCTTTTTTTTTTTTT
