Hartwell's complete (HC) medium

10x HC dropout solution, per liter, autoclave

• Methionine 200 mg
• Tyrosine 600 mg
• Isoleucine 800 mg
• Phenylalanine 500 mg
• Glutamic acid 1000 mg
• Threonine 2000 mg
• Aspartic acid 1000 mg
• Valine 1500 mg
• Serine 4000 mg
• Arginine 200 mg

10x YNB, per liter, autoclave

• Yeast nitrogen base (without amino acids or ammonium sulfate) 14.5 g
  • Note: this results in 15% less of the vitamin/salt mix than Difco YNB w/o amino acids.
• Ammonium sulfate 50 g

• Alternatively, Yeast nitrogen base (without amino acids) 57.1 g
• Ammonium sulfate 7.4 g

1x amino acid solutions, separate, autoclave, per liter

• Uracil 1 g (0.5 g in 500 ml)
• Adenine 1 g (0.25 g in 250 ml)
• Lysine 10 g (2.5 g in 250 ml)
• Tryptophan 10 g (1.0 g in 100 ml)
  • keep in light blocking bottle
• Leucine 20 g (2.0 g in 100 ml)
• Histidine 10 g (1.0 g in 100 ml)

Final HC medium

• Autoclave 20 g of agar in 619 ml water
• Add 100 ml 20% sterile glucose solution
• Add 100 ml 10x YNB solution
• Add 100 ml 10x HC dropout solution

Optionally add

• Adenine solution 20 ml
• Lysine solution 12 ml
• Leucine solution 4 ml
• Histidine solution 2 ml
• Tryptophan solution 8 ml
• Uracil solution 35 ml

Bring the final volume to 1 liter