Godin:Protocols/Lambda DNA Aliquots

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Lambda DNA Aliquots

In order to avoid contamination of the stock solution of Lambda DNA, aliquots must be made.

An aliquot is created by combining the following solutions in a microcentrifuge tube:

  • 10μL of stock 500μg/mL Lambda-DNA
  • 10μL of solution containing 2 mol/L KCl, 20 mmol/L HEPES, 2 mmol/L EDTA brought to a pH of 7
  • 80μL of solution containing 1 mol/L KCl, 10 mmol/L HEPES, 1 mmol/L EDTA brought to a pH of 7

Once combined, gently mix the aliquot and store at -20°C.

To use for testing, inject 10 μL of the aliquots into the 500μL Teflon cell volume. This will result in a 1μg/mL DNA concentration for recording translocation events.