Gentamicin
Experimental
Stock Solution
10mg/ml in H20, store at -20°C (from here)
Soluble in water at least to 200 mg/ml
Insoluble in 50% alcohol
Working Concentration
20μg/ml [1]
80μg/ml for Mycoplasma selection
Mode of Action
Inhibits protein synthesis by binding to L6 protein of the 50S ribosomal subunit.
(from Molecular Cloning)
Resistance Gene
The most common resistance gene is the aacA-aphD gene found in the transposon Tn4001. It conveys resistance to both Gentamicin and Kanamycin in two separate domains. Another common resistance gene is the aacC1 gene, (conveying resistance to Gentamicin only) is found in the plasmid pJN105. [2, 3]
Safety
Gentamicin is an important antibiotic for human use. Think about alternative antibiotics when choosing to incorporate gentamicin resistance into your constructs. Particularly, think carefully about inserting gentamicin resistance into mobile plasmids or mobile transposons.
References
- Khlebnikov A, Skaug T, and Keasling JD. Modulation of gene expression from the arabinose-inducible araBAD promoter. J Ind Microbiol Biotechnol. 2002 Jul;29(1):34-7. DOI:10.1038/sj.jim.7000259 |
- Newman JR and Fuqua C. Broad-host-range expression vectors that carry the L-arabinose-inducible Escherichia coli araBAD promoter and the araC regulator. Gene. 1999 Feb 18;227(2):197-203. DOI:10.1016/s0378-1119(98)00601-5 |
- Wohlleben W, Arnold W, Bissonnette L, Pelletier A, Tanguay A, Roy PH, Gamboa GC, Barry GF, Aubert E, and Davies J. On the evolution of Tn21-like multiresistance transposons: sequence analysis of the gene (aacC1) for gentamicin acetyltransferase-3-I(AAC(3)-I), another member of the Tn21-based expression cassette. Mol Gen Genet. 1989 Jun;217(2-3):202-8. DOI:10.1007/BF02464882 |
