GECKIL:Tools of the Trade

From OpenWetWare
Jump to: navigation, search

RESOURCES

THE GECKIL GENETIC ENGINEERING LABORATORY is located in the west wing of the colossal E-shaped <a href="http://cms.inonu.edu.tr/tr/ibtam" onclick="window.open(this.href, , 'resizable=yes,status=yes,location=yes,toolbar=yes,menubar=yes,fullscreen=yes,scrollbars=yes,dependent=no'); return false;">Advanced Research Center</a> (ARC) of Inonu University. The laboratory is fully equipped to support molecular biology and genetic engineering studies. DNA amplification, gene cloning and expression, pathway and metabolic engineering are the type of rearch methodologies being carried out. Some basic tools of the trade in our lab:

  • Apparatus for gel electrophoresis for nucleic acids and protiens, gel imaging system, gel dryers, Southern and Northern blotting
  • PCR: PCR and rt-PCR
  • Chromatographic equipment (e.g., fraction collector) for protein isolation and purification, Western blotting
  • Cell culture room with laminar flow hood, CO2 incubators, inverted microscope
  • Spectrophotometry: UV-Vis spectrophotometers, nanodrop spectrophotometer, UV-Vis transilluminator,  microplate reader
  • Incubators: gyratory and static type, water baths
  • Freezers: refridges (3 –20 oC) and ultralow freezer (–80 oC).
  • Balances: coarse and fine
  • Santrifuges: microfuges, medium and high speed low-temp or ambient-temperature centrifuges
  • Owens: microwave and drying
  • Fermentors (1 liter and 2 liter)
  • Disolved oxygen monitoring system

​Some other ARC instrumentation at our disposal:

  • Multi (3) Fluorescence microplate reader 
  • Transmission electron microscopy (TEM)
  • Scanning electron microscopy (SEM)
  • NMR spectrometry
  • Elemental analysisis apparatus
  • Atomic absorption spectrometry (AAS)
  • Infrared spectrometry
  • High-pressure liquid chromatography (HPLC)
  • Gas chromatography (GC)
  • Gas chromatography/Mass spectrometry (GC/MS)
  • X-ray analysis setting
  • X-ray flourescnet spectrophotometry (XRF)
  • Inductively-coupled plasma system (ICP)
  • Liquid nitrogen genertion system