Freimoser:Recipes:TransferBuffer

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Transfer Buffer

Final working concentration (1x)

  • 3 g Tris
  • 14.4 g Glycine
  • 800 mL H2O
  • 200 mL Methanol

10x stock solution

  • 30 g Tris
  • 144 g Glycine

Fill up to 1000 mL with H2O.

To make the 1x working solution from this stock use:

  • 100 mL 10x stock solution
  • 700 mL H2O
  • 200 mL Methanol