Freimoser:Recipes:TransferBuffer
From OpenWetWare
Jump to navigationJump to search
<html><style type='text/css'> A:link {
text-decoration: underline; font-size: 10pt; font-style: normal; font-family: arial, trebuchet ms; color: #003399;
}
A:visited {
text-decoration: underline; font-size: 10pt; font-style: normal; font-family: arial, trebuchet ms; color: #003399;
} A:active {
text-decoration: none; background-color: #99ccff; color: #660099;
}
A:hover {
text-decoration: none; color: #ff0033;
}
.navbar{
padding: 0px; width: "100%"; font-family: Arial; font-size: 10pt;
} </style></html>
Transfer Buffer
Final working concentration (1x)
- 3 g Tris
- 14.4 g Glycine
- 800 mL H2O
- 200 mL Methanol
10x stock solution
- 30 g Tris
- 144 g Glycine
Fill up to 1000 mL with H2O.
To make the 1x working solution from this stock use:
- 100 mL 10x stock solution
- 700 mL H2O
- 200 mL Methanol