Freimoser:Recipes:NZY

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NZY medium

Composition (for 1L)

  • 10g Yeast extract
  • 5g NaCl
  • 2g Glucose
  • 16g NZ-Amine A

Fill up to 1 liter and adjust to pH 7.

Comment

  • We use this medium to grow bacteria if purification on amylose resin (with MBP tag) is sought
  • The use of NZ-amine A also lactose free (in contrast to bacteriological peptone)
  • NZ-amine A is available from Sigma (C0626-250g)