The Free Genes Project has several DNA synthesis projects. Here are breakdowns of each.
GLOBAL ASSUMPTIONS
- It takes 2 sequencing reactions to sequence an average gene (1 build well)
- A 96-well build to completion takes 120 minutes of active labor
- In each project, 1/12 of the constructs fail, requiring more builds + sequencing
- Assembly and transformation costs $2.50 in reagents. Sequencing costs $3.25 (per deal with Twist)
- An average plate contains 100kbp of sequence
- By this logic, each 100kbp costs $936 and 130 minutes of labor
- Labor is not factored into costs (but if it was, it would only raise the cost by $43.5)
- It takes 360 minutes to set up the bioinformatics pipeline to do any of these tasks.
Completed Projects
NOTHING HERE
Undergoing Projects
E.coli JCVI-Syn3.0 genes
- Synthesize all JCVI-Syn3.0 genes codon optimized for E.coli
- Assists investigations of how to build-a-cell from scratch
Breakdown
| Base Pairs
|
500kbp
|
| Labor
|
16.8 hours
|
| Other Costs
|
$4,680
|
Yeast Wolbachia genes
- Synthesize all Wolbachia genes
- Wolbachia genes may prevent Zika and other dangerous viruses in Mosquitos
Breakdown
| Base Pairs
|
1500kbp
|
| Labor
|
38.5 hours
|
| Other Costs
|
$14,040
|
Proposed Projects
Ordered by most to least resource intensive
All below projects would use 5.2mbp of DNA synthesis, or 52% of our budget
iGem kit reproduction
- Completely reproduce the iGem 2018 kit
- Distribute widely under OpenMTA terms and conditions
Breakdown
| Base Pairs
|
2700kbp
|
| Labor
|
64.5 hours
|
| Other Costs
|
$25,272
|
FreeCRISPR
- Synthesize many unknown Cas9/Cpf1 - like proteins
- Empower a distributed network of people to characterize CRISPR proteins for educational purposes
Breakdown
| Base Pairs
|
1000kbp
|
| Labor
|
27.6 hours
|
| Other Costs
|
$9,360
|
The Essential Set
- Synthesize E.coli and B.subtilis essential gene sets
- A useful set of genes for a "lineage agnostic" cell
Breakdown
| Base Pairs
|
800kbp
|
| Labor
|
23.3 hours
|
| Other Costs
|
$7,488
|
Top 100 Addgene plasmids
- Synthesize top 100 Addgene plasmids to create instant charismatic content for bionet
- Publicizes more widely OpenMTA
Breakdown
| Base Pairs
|
600kbp
|
| Labor
|
19 hours
|
| Other Costs
|
$5,616
|
Yeast ribosomes
- Synthesize all known ribosomal parts for the yeast ribosome and mitochondrial ribosome
- Allows potential for Eukaryotic / mitochondrial PURE
Breakdown
| Base Pairs
|
120kbp
|
| Labor
|
8.6 hours
|
| Other Costs
|
1,123
|
