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- An expensive and fragile cuvette
You need at least 120 μL for a good reading; dilute into water appropriately (eg. 20x dilution is 6 μL sample in 114 μL water). Also if you are reading DNA or other sample with a buffer, use a similar buffer at the same concentration in preparing your blank.
- If the spec's in lamp-off mode, press any button with your finger.
- Press "Esc" until you reach the main menu.
- Select the type of reading you are looking for.
- Enter the correct λ for your sample
- Add at least 120 μL of your prepared blank to the cuvette.
- Measure the blank and thoroughly rinse out the cuvette.
- Add at least 120 μL of your sample to the cuvette.
- Use the measured absorbance to calculate concentration.
Take your absorbance at λ and multiply it by the dilution factor and the constant for the sample in question (e.g., CDNA
= 50) The number obtained is the concentration in μg/mL.