Fong:IPTG

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Preparation of 1M IPTG stock

  1. Dissolve 2.83g of IPTG in 10mL of pure water (in a clean, small beaker)
  2. Filter sterilize with syringe filter and deposit into clean 50mL conical tube
  3. Split into 1mL aliquots and store in -20C freezer

Notes

  • When adding to 5mL overnight culture, add 5μL of stock solution for 1mM final concentration.
  • When adding IPTG to LB agar plates, a typical concentration is 0.1-0.5mM IPTG.