Fluorescein diacetate
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- fluorescein (494/518) is formed by intracellular hydrolysis of FDA
- bacteria can hydrolyze this
- leaks from cells
- fluorescein has a relatively high rate of photobleaching
- pH-sensitive fluorescence ref (pKa ~6.4) that is significantly reduced below pH 7
- relatively broad fluorescence emission spectrum (not good for multicolor use)
