File:1a ch1 FRET.tif

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Name: FRET Claw FRET Fluorescence w/ 532nm laser LPFR filter Date: October 9, 2013 Gel type: Agarose gel Percentage: 2% gel Buffer: 0.5X TBE + 11 mM MgCl2 Voltage: 44 volts Length of gel run: 18.5 hours Temp: RT ~25 C BC = Blunt Claw AC = Poly A Claw A = Acceptor D = Donor

Lanes (L to R): 1 Ladder (1/48 diluted) (Not visible) 2 BC + D 0.1 pmol 3 BC + D 0.2 pmol 4 BC + A 0.1 pmol 5 BC + A 0.2 pmol 6 BC + AD 0.1 pmol 7 BC + AD 0.2 pmol 8 AC + D 0.1 pmol 9 AC + D 0.2 pmol 10 AC + A 0.1 pmol 11 AC + A 0.2 pmol 12 AC + AD 0.1 pmol 13 AC + AD 0.2 pmol

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current13:55, 22 October 2013Thumbnail for version as of 13:55, 22 October 2013781 × 1,357 (4.04 MB)Moorsalin Munshi (talk | contribs)Name: FRET Claw FRET Fluorescence w/ 532nm laser LPFR filter Date: October 9, 2013 Gel type: Agarose gel Percentage: 2% gel Buffer: 0.5X TBE + 11 mM MgCl2 Voltage: 44 volts Length of gel run: 18.5 hours Temp: RT ~25 C BC = Blunt Claw AC = Poly A Claw A =

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