FLP

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The 2micron plasmid in Yeast Sacharomyces cerevisiae

General

  • each yeast cell contain about 100 copies of the 2micron plasmid
  • 2micron plasmid replicate autonomously
  • exist in 2 isomers inside each cell, each about 50% .

FLP

  • FLP invert a sequence between 2
  • FLP mediated recombination plays an important role in plasmid amplification
  • Excessive amplification is toxic for the cell, so FLP is tighly controlled

FRT site

  • 3 repeat of 13 bp (a, b, c)
  • 8bp (AT)-rich core region, with assymetric sequence, dictate directionnality.
  • assymetric distribution and orientation of abc sites arround the core sequence:

-----c-----→ -----b-----→ ≤core≥ ←------a-------