Escherichia coli/Methylation

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  • Type I methylation systems:
    • E. coli K12 restricts DNA which is not protected by adenine methylation at sites AA*C[N6]GTGC or GCA*C[N6]GTT, encoded by the hsdRMS genes(EcoKI). Deletions in these genes removes either the restriction or methylation or both of these functions.
    • E. coli B derivative strains contain an hsdRMS system (EcoBI) restricting and protecting the sequence TGA*[N8]TGCT or AGCA*[N8]TCA.
  • The mcrA gene (carried on the e14 prophage) restricts DNA which is methylated in CmCWGG or mCG sequences (methylation by the dcm gene product).
  • The mcrBC genes restrict RmC sequences.
  • The mrr gene product restricts adenine methylated sequences at CAG or GAC sites.
  • E. coli methylates the adenine in GATC (and the corresponding A on the opposite strand) with the dam gene product.
  • M.EcoKII methylates the first A at the palindromic site ATGCAT (as well as the corresponding A on the opposite strand), see (Kossykh VG (2004) J. Bact 186: 2061-2067 PMID 15028690) Note that this article has been retracted; the retraction appears to center on textual plagarism, not experimental results. The homology to AvaIII is real. I think I believe it. tk 20:28, 9 December 2005 (EST). Rich Roberts reports: "We have tried ourselves to detect activity with this gene product and cannot detect any methyltransferase activity. In our case we used antibodies able to detect N6-methyladenine or N4 methylcytosine in DNA. The ones we have are very sensitive and should have been able to detect 5-methyl groups in the whole E. coli chromosome. Nothing was detected in an over-expressing strain."
  • For additional information see E. coli restriction-modification system and the NEB technical information on methylation.