Endy:Victor3 plate reader/Plates

From OpenWetWare

The Endy Lab uses plates made by Greiner Bio One.

Path length as a function of volume added to a well

Calculated from the volume of a frustrum using the plate dimensions given in the link above.

Volume (µl) Path Length (cm)
50 0.15
100 0.29
150 0.44
200 0.58
250 7.2
300 0.85
350 0.99
400 1.12

Converting absorbance of a 200μl sample to OD600 using the path length calculated above yields very different numbers than when using a calibration produced from samples measured in both the Endy Lab portable spec and the plate reader.