Endy:Mycoengineering Subgroup
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Mycoengineering Weekly Task List
Week of 7/24/17
"Rolando"
- Build pSB3K5_sfGFP_AgroT and transform into Agro., image cells
- Run G. lucidum ATMT
- Retreat poster
- Quals packet
- Test PLL treated cover slip fungi cell immobilization
- Test nanopipette injection of immobilized fungi cells
Week of 7/24/17
"Rolando"
- build vector with sfGFP driven by 5' UTR of virE. (should report when I am sufficiently inducing the A. tum. Type IV secretion system).
- Prepare for meeting with X.
last week:
- advised REUs.
- identified suitable standard vector (sfGFP driven by J23119) to transform into A. tum.
- designed and ordered primers/REs for building pHg_GL_EXP backbone, pHg_GL_virEsfGFP.
Week of 7/17/17
"Rolando"
Build "vir" induction reporter vector.
- tansforma agro.
- build vector with gfp into CAMBIA based vector
last week:
spent my time advising/mentoring REUs
Week of 7/10/2017
Rolando
Build vir induction reporter vector.
- Transform Agro. with E. coli GFP/RFP reporter vector.
- Build vector with GFP driven by vir gene promoter.
Run electroporation experiment (w/Isaac).
- Electroporate pHg_LB, pHg_GL and pCbx.
Protoplasts (w/Isaac).
- Design protoplast optimization (w/close attention to regeneration).
- Run experiment.
Isaac
Last week:
Sequence Verifying pHg and pCbx mycobricks
Protoplasting and plating G. lucidum
Starting liquid and plated cultures
Prototyping a climate recording grow box
This week:
Protoplasting G. lucidum for transformation and troubleshooting
Electroporation of G. Lucidum for transformation
Agrobacterium mediated transformation of G. lucidum
Design of pHg_GFP and pHg_Cas9 plasmids