Endy:Lab Meeting 7/27/2005
- Great 2005 LabRetreat
- SuperGroup: Yes or No?
- codon shuffle mRFP -- idea here is to get rid of any "cryptic" RNaseE sites
- compute expected secondary structure of 5' end as a function of FP ordering (mFOLD)
- measure mRNA levels
- use RNaseIII to process (i.e., cleave) primary transcripts. grab R0.3 from T7. talk to Berkeley iGEM student (see DE)
- run with full media (not just buffer) through the elutriator