NEBuffer EcoR I
- Improving the efficiency of EcoRI/SpeI Double Digest.
- Reaction Volumes - I used to have very inconsistent results when digesting with EcoRI, using the 20μl reaction volume. Using the Knight Lab's 50μl mix has recently given good results. I haven't tested this enough to say it is a statistically significant result. --BC 13:39, 2 Jun 2005 (EDT)
- The custom EcoRI buffer provided by NEB contains Triton-X100. This detergent is very inhibitory in transformations, so direct ligation and cloning of DNA cut in this buffer is problematic. Remarkably small amounts of this buffer dramatically reduces transformation efficiency.
- The FastDigest restriction enzymes from Fermentas all work very efficiently in one universal buffer. I perform digests in a 30 μL reaction volume. *Karmella Haynes 14:07, 19 January 2012 (EST):
- Morrow-PNAS-1972 pmid=4343967
// Discusses cleavage of DNA at a unique location by EcoRI
- Mulder-PNAS-1972 pmid=4343959
// Specificity of EcoRI
- Hedgpeth-Proc-Natl-Acad-Sci-USA-1972 pmid=4343974
// Identifies the overhang sequence produced by EcoRI
- Bigger-Nat-New-Biol-1973 pmid=4578426
// No abstract available online.
- Mertz-Proc-Natl-Acad-Sci-USA-1972 pmid=4343968
// Use of EcoRI to generate DNA fragments that can be ligated
- Cohen-PNAS-1973 pmid=4594039
// Use of EcoRI to generate recombinant DNA fragments </biblio>