Eccles:genotyping oligos
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Eccles Lab > DGG Oligos > genotyping_oligos >
MOUSE
KRD PRIMERS
- Organism: Mus Musculus
- Forward Primer: CTGCCGGAAATCGTCGTGGTATTCACTCCA (30 bp)
- DGG ID:
- DGG name: CATF
- Current [stock]:
- Reverse Primer: CCAGACCGTTCAGCTGGATATTACGGCCTT (30 bp)
- DGG ID:
- DGG name: CATR
- Current [stock]:
- Annealing Temperature: 72 °C
- approx 250 bp
- Amplifies a portion of the CAT transgene
GFP PRIMERS
- Organism: Mus Musculus
- Forward Primer: AGC GCG ATC ACA TGG TCC TG (20 bp)
- DGG ID:
- DGG name: GFPF
- Current [stock]:
- Reverse Primer: ACG ATC CTG AGA CTT CCA CAC T (22 bp)
- DGG ID:
- DGG name: GFPR
- Current [stock]:
- Annealing Temperature: 54°C
- 321 bp
- Forward primer in the eGFP cDNA and the reverse is in the globin sequence
- Programme - Jody - GFP on red machine
MEST PRIMERS
- Organism: Mus Musculus
- Forward Primer: AAGACGGAGGTGTGCTTTCC (20 bp)
- DGG ID:
- DGG name: Mest8F
- Current [stock]:
- Common Primer: TGTCGATGACCAGGTTGCC (19 bp)
- DGG ID:
- DGG name: MEST9R
- Current [stock]:
- Reverse Primer: GCATCGCCTTCTATCGCCTT (20 bp)
- DGG ID:
- DGG name: MEST10FJ
- Current [stock]:
- Annealing Temperature: 58 °C, 1 minute extension
- 8F and 9R bind to exon 8 and 9 of the wildt ype Mest gene and give a PCR product of 314 bp (wildtype). The mutant band is larger.
- Programme, on Red machine - Jody - Mest
Pax2-1Neu
- Forward Primer: gggcacgggggtgtgaaccag
- DGG ID: 2292
- DGG name: P2PB1F
- Current [stock]:
- Reverse Primer: ctgcccaggattttgctgacacagcc
- DGG ID: 2293
- DGG name: PAX2EXTR
- Current [stock]:
- Amplicons: 167 (wt), 168 (mutant)
