Eccles: DGG Protocols
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Nucleic acid
- Total RNA isolation with Tri Reagent and columns (Aaron)
- cDNA synthesis (Jess Protocol)
- DNA isolation (Jody Protocol)
- PCR product sequencing (Matt Protocol)
- qPCR reaction (Jess Protocol)
- Random oligo hyb (Aaron)
- Bioanalyzer (Aaron)
Cell culture
- CO2 for cell culture
- siRNA transfection (Jess Protocol)
- siRNA forward transfection with Lipofectamine 2000 (Aaron)
- siRNA reverse transfection with Lipofectamine 2000 (96-well) or RNAiMAX (24-well) (Aaron)
- Antisense transfection (Jess Protocol)
- Culturing NZM cell lines (Jess Protocol)
- Making primary cells from mouse tissue (Lana)
- Making primary cells from surgery tissue (Lana)
- Culturing CB cells (Mouse Embryonic Kidney Cells, SV40 large T positive) (Lana)
- Collagen coating of cell culture vessels (Lana)
- Culturing human ADPKD primary kidney cells (Lana)
- MTT Assay (7d) on CB Cells (Lana)
- Eccles:MKS Epithelial Cell Culture (Lynn)
Protein
- Western Blotting (Lana)
- Western Blot Stripping (Lana)
- Protein lysates from cells in culture (Lana)
- Protein lysates from tissue (Lana)
- MITF western protocol (Lynn)