ESSCOSMOS/2009:Enzymes Soil & Fungal Enzymes

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COSMOS Summer 2009:

Global Change Chemistry & Biology

Prairieroad.jpg

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Overview

In this activity, we will study the organisms and environment along a gradient from the edge of a marsh to the top of the hill.

Objectives

  • Investigate the relationship between enzyme activity and CO2 release from substrate being decomposed by different species of mushrooms.
  • Create graphical summaries of the findings
  • Enter data into class database

Background

Experimental protocol

We will be using a colorometric method developed by Dr. Steve Allison to measure the activity of enzymes in substrate that it colonized by mushroom mycelia.

We will follow the methods outlined here: experimental protocol

Graphical summary

Analysis

Homework