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Workshop on Molecular Biology and Protein Purification

Poster for workshop

Introduction

There is currently a high demand for fuels, medicines and other fine chemicals to meet the needs of the growing world population. Conventional production of these commodity chemicals is costly and also requires a considerable amount of effort to obtain yields enough to meet the global demand. For example, production of pharmaceuticals from plant sources requires the harvesting of several plant materials and using an expensive extraction process which in effect adds to the cost of production. Using synthetic biology, some of these challenges can be overcome. With synthetic biology tools, DNA fragments (promoters, genes, terminators, etc) can be synthesised (or cloned), assembled into synthetic biological ‘circuits’ and overexpressed in a host cell (chassis). These DNA fragments can be standardised into pieces called BioBricks such that they contain an EcoRI+XbaI restriction sites upstream of the DNA fragment (BioBrick prefix) and an Spe+PstI restriction site downstream of the fragment (BioBrick suffix). This allows easy assembly of BioBricks using any of the BioBrick assembly protocols. With this concept, it will be possible to clone and overexpress non-native genes in a heterologous host such as E. coli or S. cerevisiae to obtain high amounts of the desired product following optimisation. Biological circuits engineered with synthetic biology tools can also be made to sense different input signals and produce an output. An application of this is the development of biosensors which can detect signals such as mercury in polluted water and produce a measurable desired output.

Activities

Topics to be covered in this workshop are

  1. PCR design and optimization
  2. Expression and purification of Taq and pfu DNA polymerases
  3. Cloning & protein engineering
  4. DNA fingerprinting & metagenomics
  5. Gel analysis with ImageJ

Date

21st - 25th September, 2015

Duration

One week

Short Course on Public Health Applications of Generic engineering

Introduction

The course will cover theoretical principles and some modern practical techniques for public health applications of genetic engineering.

Activities

Lectures Practicals

Details coming soon