Dspelke- Module 1: Genome Engineering
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M13 Genome
| Protein | Function | Re-engineering Ideas |
|---|---|---|
| I | Assembly | Modifiy the gene so that the channels becomes less selective and thus allow other ions, molecules, and proteins to enter and exit the bacteria- see how this affects the life cycles of both the bacteria and the phage |
| II | Replication of DNA + strand | Alter the gene to make it more active and thus replicate DNA more frequently- see how increased DNA production affects phage growth |
| III | Phage tail protein (5 copies) | Modify the proteins that bind to the bacteria (and thus initiate the F pilus and infection) so that the bacteriophage canbind to and infect other types of bacteria- examine the varied life cycles that result |
| IV | Assembly | Alter the gene in such a way as to destabalize the outer membrane (e.g. no longer detergent-resistant)- test varying environments for phage survival rate |
| V | Binds ssDNA | Vary the activity of the gene and thus the competition between dsDNA formation and the sequestering of ssDNA- compare the results to find the optimum level of phage production possible |
| VI | Phage tail protein (5 copies) | Add some sort of tag to the gene that is only visible when p6 is outside of the bacteria- thus we would be able to determine when the phage has been secreted |
| VII | Phage head protein (5 copies) | Alter the gene so that p8 cannot be substituted for p5- see how this affects the phage (e.g. can it still be secreted?) |
| VIII | Phage coat protein (2700 copies) | Add a small protein to the gene that we would like to amplify becuase p8 is synthesized so many times- see if this method works and if yes, what applications could this be used for? |
| IX | Phage head protein (5 copies) | Modify the gene so that it can bind to bacterial surface proteins (like p3 does)- see if this allows the phage to interact with other bacteria (now that both ends can bind) |
| X | DNA replication | Altering this gene will also alter gene 2 so any alteration would affect both genes, so make any number of small modifications - see what interesting phenomena result |
| XI | Assembly | Modify the gene so that it is longer, hopefully resulting in a larger channel- see if this could allow multiple phages to pass through, thus making the channels more effective |
