Design 4

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Pre-Working Stocks

Description Contents Total
c4.0.4L: barrel oligos @ outside aptamers + ligand 4.0.4.1oa/ob, 4.0.4.2oa/ob, 4.0.4.3oa/ob, 4.0.4.4oa/ob 8
c4.0.6L: barrel oligos @ inside aptamers + ligand 4.0.6.1oa/ob, 4.0.6.2oa/ob, 4.0.6.3oa2/ob2, 4.0.6.4oa2/ob2 8

(mix 5 μL of each 100 μM stock ligand-oligos to make pre-working stocks)


Working Stocks

Working Stocks Description Pre-Working Stocks Water Total
c4.0FL.core latch design 1, inside aptamers 1 (66 μL), 2 (33 μL), 3 (33 μL), 4 (4 μL), 6L (8 μL) 48 μL 200 μL
c4.0FL.latches latch design 1, inside aptamers 8 (4 μL), 11 (2 μL), 12 (2 μL) - -
c4.0GL.core latch design 1, outside aptamers 1 (66 μL), 2 (33 μL), 3 (33 μL), 4L (8 μL), 6 (4 μL) 48 μL 200 μL
c4.0GL.latches latch design 1, outside aptamers 8 (4 μL), 11 (2 μL), 12 (2 μL) - -
c4.0HL.core latch design 2, inside aptamers 1 (66 μL), 2 (33 μL), 3 (33 μL), 4 (4 μL), 6L (8 μL) 40 μL 200 μL
c4.0HL.latches latch design 2, inside aptamers 9 (4 μL), 13 (2 μL), 14 (2 μL), 23 (4 μL), 24 (2 μL), 25 (2 μL) - -
c4.0IL.core latch design 2, outside aptamers 1 (66 μL), 2 (33 μL), 3 (33 μL), 4L (8 μL), 6 (4 μL) 40 μL 200 μL
c4.0IL.latches latch design 2, outside aptamers 9 (4 μL), 13 (2 μL), 14 (2 μL), 23 (4 μL), 24 (2 μL), 25 (2 μL) - -