Davidying: SAGA Subunits
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SAGA Protein Subunit Descriptions
SAGA subunits, S. cerevisiae
| Ada subunits | size,chromosome,null p-type | notes |
|---|---|---|
| Ada1 (aka HFI1, SUP110, SRM12, GAN1) | 1.467 kb/489 aa, Chr. XVI, viable |
adds structural integrity to SAGA Complex, when deleted mutants show reduced fitness in rich media and decreased metabolite accumulation, growth defect with non-fermentable carbon source |
| Ada2 (aka SWI8) | 1.305 kb/434aa, Chr. IV, viable |
transcription coactivator, when deleted mutants show reduced fitness in rich media, increased metabolite accumulation (glycogen), decreased drug resistance |
| Ada3(aka NGG1, SWI7) | 2.109 kb/702aa, Chr. IV, viable |
transcriptional regulator, decreased drug resistance, reduced fitness in rich media |
| Gcn5 (aka ADA4, SWI9) | 1.32 kb/439aa, Chr. VII, viable |
Histone acetyltransferase, catalytic subunit of ADA and SAGA, when deleted showed reduced fitness in rich media |
| Ada5 (aka SPT20) | 1.815 kb/604aa, Chr. XV, viable |
subunit of SAGA transcriptional regulatory complex, adds structural integrity, when deleted mutants show decreased drug resistance and and decreased metabolite accumulation |
| Spt subunits | size, chromosome, null p-type | notes |
|---|---|---|
| Spt3 | 1.014 kb/337aa, Chr. IV, viable |
interacts with Spt15p to activate transcription of some RNA polymerase II-dependent genes, also functions to inhibit transcription at some promoters, when deleted show reduced fitness in rich media, show decreased resistance to wortmannin and increased resistance to rapamycin, decreased metabolite accumulation |
| Spt7(aka GIT2) | 3.999 kb/1332aa, Chr. II, viable |
involved in proper assembly of SAGA complex, increased drug resistance to rapamycin, decreased to wortmannin |
| Spt8 | 1.809 kb/602aa, Chr. XII, viable |
required for SAGA-mediated inhibition at some promoters, same thing about drug resistance, decreased metabolite accumulation |
| Spt20 (aka Ada5) | 1.815 kb/604aa, Chr. XV, viable |
adds structural integrity to SAGA Complex, same thing about drug resistance, decreased metabolite accumulation |
| TAF subunits | size, chromosome, null p-type | notes |
|---|---|---|
| TAF5 (aka TAF90) | 2.397 kb/798aa, Chr. II, inviable | involved in RNA polymerase II transcription initiation and in chromatin modification, dead |
| TAF6 (aka TAF60) | 1.551 kb/516aa, Chr. VII, inviable | see above |
| TAF9 (aka TAF17) | 0.474 kb/157aa, Chr. XIII, inviable | see above |
| TAF10 (aka TAF23, TAF25) | 0.621 kb/206aa, Chr. IV, inviable | see above |
| TAF12(aka TAF61, TAF68) | 1.620 kb/539aa, Chr. IV, inviable | see above |
| Tra1 subunit | size, chromosome, null p-type | notes |
|---|---|---|
| Tra1 | 11.235 kb/3744aa, Chr. VIII, inviable | interacts with acidic activators to initiate transcription, dead |
| other subunits | size, chromosome, null p-type | notes |
|---|---|---|
| Sgf73 | 1.974 kb/657aa, Chr. VII , viable |
involved in formation of the preinitiation complex assembly at promoters, decreased metabolite accumulation, reduced fitness in rich media |
| Sgf29 | 0.779 kb/259aa, Chr. III, viable |
function unknown, pH sensitive after 8 generations, reduced fitness in rich media |
| Sgf11 | 0.3 kb/99aa, Chr.XVI, viable |
regulates transcription of a subset of SAGA-regulated genes, required for the Ubp8p association with SAGA and for H2B deubiquitylation, when deleted showed reduced fitness in rich media |
| Ubp8 | 1.416 kb/471aa, Chr. XIII, viable |
Ubiquitin-specific protease that is a component of the SAGA (Spt-Ada-Gcn5-Acetyltransferase) acetylation complex; required for SAGA-mediated deubiquitination of histone H2B, reduced fitness in rich media |
| Sus1 | gene with intron, Chr. II, viable |
Protein involved in mRNA export coupled transcription activation |
