Davidying: Ideas for M13 Renovation
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M13 Redesign Ideas
| Gene | Function | Re-engineering ideas |
|---|---|---|
| g1 | assembly | Increase size but maintain shape and interactions, to attempt to create a larger "barrel" to make a wider filament of M13 |
| g2 | replication of DNA + strand | Separate g2 and g10 by inserting entire sequence of g10 after transcription end of g2/g10 (essentially repeating g10 twice in succession), so that the two genes are independent |
| g3 | phage tail protein | Change amino acid sequence to make p3 bind to other proteins besides TolA (perhaps allowing it to infect more bacteria) |
| g4 | assembly | (see g1) |
| g5 | binds ssDNA | Add GFP or other type of tag to enable rapid measurement of DNA replication (an assay of g2/g10) |
| g6 | phage tail protein | Change sequence to allow for easier modification of p3 |
| g7 | phage head protein | Change sequence to allow for easier modification of p9 |
| g8 | phage coat protein | Change amino acid sequence to allow g8 (and thus the entire virus) to bind to certain materials, like metals |
| g9 | phage head protein | Add or change residues to enable p9 to bind to p3, creating long chains of connected filaments like a nanowire |
| g10 | DNA replication | (see g2) |
| g11 | assembly | (see g1) |
