Davidson:Protocols

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Wet Lab Protocols

  1. Common molecular reagents
  2. Standard Assembly
  3. Compatibility of Plasmids
  4. Building dsDNA with Oligos
  5. Setting up PCR mixtures
  6. PCR and Mg2+ concentration
  7. Clean and Concentrate DNA (after PCR, before digestion)
  8. Pouring an agarose gel
  9. Calculate MWs
  10. Digest DNA with restriction enzymes
  11. Double Digest Guide
  12. Ethanol Precipitate DNA (short protocol)
  13. 1kb MW markers
  14. Shrimp Alkaline Phosphatase
  15. Qiagen QIAquick Gel Purification
  16. Qiagen QIAquick Column Regeneration Protocol
  17. ElectroElute Gel Purification
  18. Ligation Protocol
  19. Heat Shock Transformation OR Short version of Heat Shock
  20. Zippy Transformation
  21. Colony PCR to Screen for Successful Ligations
  22. Promega miniprep
  23. Choices for Transformation: Heat Shock vs. Zyppy
  24. Choices for Mini-Preps: Promega vs. Zyppy
  25. Colony PCR
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