DYT

1.7- DYT medium For 500 mL

• 8.0 g tryptone
• 5.0 g yeast extract
• 2.5 g NaCl

For 100 mL

• 1.6 g tryptone
• 1.0 g yeast extract
• 0.5 g NaCl

• mix in DW
• add 10 N NaOH (about 2.5 drops for 100 ml)
• check that pH=7.0 with pH paper; adjust as needed
• aliquot: add 3 ml to 15 ml glass tubes, cap loosely
• autoclave in a rack in a tub with 4 cm of water
• store at 4°C