From OpenWetWareJump to navigationJump to search
Prepared DNA ladder
- NEB's 2-log ladder is a good choice for most samples.
- Deionized Water
- Loading dye
Mix NEB 2-log ladder with deionized water to a 100 ng/μL dilution. Add loading dye and store at 4°C. Load 12 μL per lane for 1 μg of DNA.
To make 1.2 mL prepared ladder, combine:
- 900μL deionized water
- 100μL 2-log ladder (at 1μg/μL)
- 200μL loading dye