DNA ladder

From OpenWetWare
Jump to navigationJump to search

Prepared DNA ladder

Mix NEB 2-log ladder with deionized water to a 100 ng/μL dilution. Add loading dye and store at 4°C. Load 12 μL per lane for 1 μg of DNA.

To make 1.2 mL prepared ladder, combine:

  1. 900μL deionized water
  2. 100μL 2-log ladder (at 1μg/μL)
  3. 200μL loading dye