DNA Precipitation protocol

<html> <h2>Solutions/reagents:</h2><ul type="circle"><li>3M Sodium Acetate solution</li><li>Glycogen</li><li>95% EtOH</li><li>70% EtOH</li><li>water</li><li>buffer</li><li>DNA sample</li></ul><h2>Equipment:</h2><ul type="circle"><li>Centrifuge</li></ul><h2>Steps:</h2><ol><li>Measure out 0.1 volume 3M Sodium Acetate solution into DNA sample. </li><li>Measure out 1 µl of Glycogen into Eppendorf tube (1). </li><li>Add 2 volumes 95% EtOH. </li><li>Option 1: Store at -20°C for 12 hrs(overnight). (or) Option 2: Store at -80°C for 30 mins. </li><li>Centrifuge at maximum speed for at least 15 mins at room temperature, gently aspirate out the supernatant and discard it. </li>(Optional) Add 1 ml of 70% EtOH. Store at room temperature for 5 mins. </li>(Optional) Centrifuge at maximum speed for 5 mins at room temperature, gently aspirate out the supernatant and discard it. </li><li>Dry the pellet in air for 10 - 15 mins. Dry until all the liquid is gone. </li><li>Option 1: Add water to pellet. (or) Option 2: Add buffer to water. Resuspend pellet by vortexing/by shaking vigorously. </li></ol>TOTAL TIME REQUIRED FOR THE COMPLETION OF THE PROTOCOL :~ 12 hrs, 25 mins</html>

DNA Precipitation protocol

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