DIYbio:Notebook/Open Gel Box 2.0/Power Supply/Typical Setup
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- Typical Power Supply Setup in NW Lab
- 1.2% gel is good for 100bp - 5kb (but by experience I would run 2% gel for 100-500bp)
- The run time for 96v at 84mA for a 1000bp (see reference for typical prokaryote gene size I currently work with) band takes about 20 minutes to resolve, up to 40 minutes to get a good separation if you have multiple near-by bands.
- 60V gets very good separation and clean looking 1kb bands, 120V runs fast but doesn't come out as well.
Select Typical Agarose Gel Concentrations†
Agaros Conc. | Resolution Range | Run Length |
---|---|---|
0.8% | 800 bp – 10 kb | 5.8 cm |
1% | 400 bp – 10 kb | 3.2 cm |
1.2% | 100 bp – 5 kb | 5.8 cm |
2% | 100 bp – 2 kb | 5.8 cm |
4% | 20 bp – 500 bp | 5.8 cm |
† Invitrogen http://tools.invitrogen.com/content.cfm?pageid=11229