Much of our computational needs are facilitated through dedicated nodes on Oregon State University's Center for Genome Research and Biocomputinghigh-performance computing cluster. We currently own the following resources:
- pine1 - The original. Dual quad core 2.66 GHz Intel processors with 32 GB of RAM.
- pine2 - Dual quad core 2.13 GHz Intel processors with 96 GB of RAM.
- smokey - 20 TB RAID system.
These systems are currently run through a 64 bit version of Enterprise Red Hat Linux.
Solexa barcode sorting
Most of our Solexa runs include multiplex massively parallel sequencing (MMPS). Because these micro-reads include a sample-specific barcode (as well as the quality control 'T') a first step is to sort these reads by barcode and to remove the barcode. This is facilitated by a custom perl script.
- Nucl. Acids. Res. - Article describing barcoding.
- Short read toolbox - Includes barcode sorting script.
--- notice! --- As of summer 2011, we have been increasingly moving to Illumina's index sequencing method. Our experience to date from ~50 libraries has been very positive, and we are starting to explore novel indexes beyond the currently-available list of 24.