Crisanti:Materials and Orderforms

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DNA & Cloning

PCR

• Taq DNA Polymerase
  

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• BD Advantage 2 PCR Kit
  

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• Phusion™ High-Fidelity DNA Polymerase
  

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• HS Phusion™ High-Fidelity DNA Polymerase
  

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• MWG Primers
  

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• Fast SYBR® Green Master Mix
  

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• PCR strip tube separate cap
  

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• ExoSAP-IT (USB)
  

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Competent Cells

• One Shot® TOP10 40x50ul
  

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• Subcloning Efficiency Commpetent Cells
  

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Enzymes

• Restriction Enzymes

• NEBs Antarctic Phosphatase
  

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• NEB T4 DNA Ligase

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• NEB VentR DNA Polymerase

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• NEB VentR (exo-) DNA Polymerase

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• Fermentas AscI FAST digest
  

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• NEB AscI digest
  

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• NEB Dpn1
  

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Cloning Kits

• pGEM®-T Easy Vector System I
  

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• pET101 TOPO Expression Kit
  

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• Zero Blunt Topo PCR cloning Kit

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• Takara DNA Ligation Kit 2.1
  

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Kits (Qiagen, Promega)

• QIAgen Plasmid Maxi Kit (25)
  

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• QIAquick gel extraction kit
  

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• Hispeed Maxi
  

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• EndoFreeHispeed Maxi
  

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• PCR purification Kit
  

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• Wizard® Genomic DNA Purification Kit
  

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• QIAprep Spin Miniprep Kit (250)
  

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RNA

• RNA free tips (1000,200,10ul) and tubes
  

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• MEGAscript® High Yield Transcription Kit 25rxn
  

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• MEGAclear® Kit 20rxn
  

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• Yeast tRNA (10 mg/ml)
  

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• SUPERase•In™ (20 U/µl) 2500u RNAse Inhibitor
  

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• NorthernMax® Formaldehyde Load Dye 6x1ml
  

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• Ambion 10 mM Bio-16-UTP
  

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• Ambion RACE Kit
  

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• Ambion Northenmax2 kit
  

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• Ambion T7 MessageMachine
  

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• Francescas Ordeform
  

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Electrophoresis

• Bioline Hyperladder 1 (200-10 000 bp)
  

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Cell Culture

• Invitrogen's FBS
  

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• Invitrogen's Scheider's Drosophila Medium
  

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• Effectene Qiagen
  

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• Easy Flasks
  

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• Penicillin-Streptomycin Solution, liquid 100ml
  

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Protein Stuff & Antibodies

• Anti-HA Tag mouse Monoclonal
  

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• Leupeptin & Antipain Protease Inhibitors
  

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Radioactive Isotopes

• α-P32 dATP

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• α-P32 UTP

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Chemicals

• Glucose (for teh Mossies)
  

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Injections

• Femtotip (from VWR)
  

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• Microloader (from VWR))

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• Microscope Slides (from VWR)
  

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• 8 well Microscope Slides (from MP Biomedicals)
  

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Microarray Group stuff

• Tyramide
  

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• Tips
  

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• Silica Gel
  

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• Microplates
  

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• Fischer Water
  

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• Cel Associates Fedex
  

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• Cel Associates
  

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• Syringe filter
  

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• Gene frames
  

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• Media Hybridoma
  

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• Flasks Hybridoma
  

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• ProteinAcolumn Hybridoma
  

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• BSA
  

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• Alexa Antibodies
  

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Blotting

• Hyperfilm ECL
  

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• Hybond ECL Nitrocellulose Membrane
  

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• Hybond N+ Roll 1m Nitrocellulose Membrane
  

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Safety and Waste

• Sharps container, for non-infectious sharps and needles
  

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Other Stuff

• illustra ProbeQuant™ G-50 Micro Columns
  

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• BD Falcon™ Cell Strainers, yellow, 100 μm
  

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• Ultrapure DNAse/RNAse-Free distilled water 500ml
  

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• Chromatography Columns, empty 50
  

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• Nalgene Filter units + Bottles, sterile 250ml
  

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• Biorad gel equipment
  

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• Slide-A-Lyzer® Dialysis Cassettes 10/pkg 7000MWCO
  

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• SilverSNAP Staining Kit for Mass Spectrometry
  

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• Dynabeads® M-280 Streptavidin 10 mL
  

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• ImmunoPure Immobilized Streptavidin, 5 ml
  

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• Colloidal Blue Staining Kit, G250 Coomassie for Masspec
  

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• Microscope Lamps UV and TM
  

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• Pinzers aka: tweezers
  

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• Snap-Cap 14ml Tubes for mini-cultures
  

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• 50ml-15ml tubes
  

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• Eppendorf tubes
  

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• Blue rolls and kimwipe
  

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• Crystal 5x DNA Loading Buffer Blue, 2x1ml (Bioline)
  

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