User:Anthony Salvagno/Notebook/Research/2009/05/07/Creation of DNA Tethers

From OpenWetWare
< User:Anthony Salvagno‎ | Notebook‎ | Research‎ | 2009‎ | 05‎ | 07(Redirected from Creation of DNA Tethers)
Jump to: navigation, search

{{#widget:Google Spreadsheet |key=rMgj4044knOqvntZrQ8sviQ|width=800|height=500}}

  • Add 1uL (5nM) of T+B adapter to tubes 6-9 every 20 min until concentration reaches 30nM.
  • Use thermocycler to keep temperature at 16C (program BBLIG on Osley PCR).